Design of Intelligent Polymer of Gelatin- Poly N-isopropyla- crylamide under Gamma radiation for Cellular Applications
| Author | Saeed Heidari-Keshel | en |
| Author | Bita Soleimani | en |
| Author | Maryam Ebrahimi | en |
| Author | Asghar Ashrafi Hafez | en |
| Author | Razieh Fallahakbarpour | en |
| Author | Mohammad Khaledian | en |
| Author | Fatemeh Yousefi | en |
| Author | Maryam Rostampour Kakroudi | en |
| Author | Forough Fathi Arateh | en |
| Issued Date | 2013-12-31 | en |
| Abstract | Thermo-sensitive polymers were prepared by graft copolymerization of gelatin with N-Isopropylacrylamide via gamma radiation. Characterization of polymers such as DSC analysis, swelling in different ratios and cell assays were investigated. DSC and solubility analysis showed gelatin and N-Isopropylacrylamide monomers were grafted via gamma radiation successfully. Results show swelling of samples increased as gelatin increased. Swelling ratio and curves results administrated hyd-ro-philicity / hydrophobicity of hydrogel that this property is due to presence of NIsopropylacrylamide in different temperatures. The polymer was tested for harvesting epithelial cells after carrying out cell culture at 37 °C and incubating the confluent cells at 10°C for spontaneous detachment of cell sheet from polymer surface without enzyme treatment. These unique properties of the hydrogel would make it a promising support for drug delivery systems and tissue regeneration. | en |
| DOI | https://doi.org/ | en |
| Keyword | Thermo- Sensitive Polymer | en |
| Keyword | Gelatin | en |
| Keyword | N-Isopropylacrylamide | en |
| Keyword | Gamma Radiation | en |
| Keyword | Epithelial Cell Sheet | en |
| Publisher | Brieflands | en |
| Title | Design of Intelligent Polymer of Gelatin- Poly N-isopropyla- crylamide under Gamma radiation for Cellular Applications | en |
| Type | Research Article | en |