Molecular Identification of Gram-Negative Bacteria in Respiratory Samples of Cystic Fibrosis Patients from a Children Referral Hospital in Tehran
| Author | Leila Azimi | en |
| Author | Roxana Mansour Ghanaiee | en |
| Author | Mehdi Shirdust | en |
| Author | Ahmad Moein Karimi | en |
| Author | Shahnaz Armin | en |
| Author | Sedigheh Rafiei Tabatabaei | en |
| Author | Farideh Shiva | en |
| Author | Fatemeh Fallah | en |
| Author | Mohammad Rezaei | en |
| Author | Sayyed Alireza Fahimzad | en |
| Author | Ghamartaj Khanbabaei | en |
| Author | Mohammad Rahbar | en |
| Orcid | Leila Azimi [0000-0002-7216-2530] | en |
| Orcid | Fatemeh Fallah [0000-0003-4455-1536] | en |
| Orcid | Mohammad Rahbar [0000-0001-8419-3791] | en |
| Issued Date | 2019-07-31 | en |
| Abstract | Background: Gram-negative bacteria are a major cause of pulmonary infection in patients with cystic fibrosis (CF). Objectives: The study aimed to conduct the molecular identification of Gram-negative bacterial flora causing pulmonary infection in children with CF. Methods: In this study, sputum samples were taken from 64 CF children undergoing treatment as outpatients or inpatients at a referral children’s hospital in Tehran. The PCR technique was used to detect the presence of Gram-negative bacteria, namely Pseudomonas spp., Acinetobacter baumannii, Stenotrophomonas maltophilia, and Burkholderia cepacia. Results: All samples were positive for 16srRNA. Pseudomonas spp. and A. baumannii were detected in 47% and 14% of the studied samples, respectively. Co-colonization by Pseudomonas spp. and A. baumannii was observed in three (5%) samples. Conclusions: According to this survey, Pseudomonas spp. were the most prevalent Gram-negative bacteria isolated from CF patients with pulmonary infection by molecular assays. | en |
| DOI | https://doi.org/10.5812/pedinfect.64834 | en |
| URI | https://brieflands.com/journals/apid/articles/64834 | en |
| Keyword | <i>Pseudomonas</i> | en |
| Keyword | Cystic Fibrosis | en |
| Keyword | Polymerase Chain Reaction | en |
| Publisher | Brieflands | en |
| Title | Molecular Identification of Gram-Negative Bacteria in Respiratory Samples of Cystic Fibrosis Patients from a Children Referral Hospital in Tehran | en |
| Type | Research Article | en |
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