Evaluation of Anticarcinogenic and Cytotoxic Effects of COX-2 Specific Inhibitors on an Animal Model of Hepatocellular Carcinoma Using Isolated Mitochondria
| Author | Alireza Mahmudi | en |
| Author | Enayatollah Seydi | en |
| Author | Nahid Ahmadi | en |
| Author | Zhaleh Mohsenifar | en |
| Author | Mahsa Azami Movahed | en |
| Author | Afshin Zarghi | en |
| Author | Jalal Pourahmad | en |
| Orcid | Afshin Zarghi [0000-0002-6470-7895] | en |
| Orcid | Jalal Pourahmad [0000-0002-4555-4168] | en |
| Issued Date | 2025-12-31 | en |
| Abstract | Background: Cancer is regarded as one of the most significant health concerns in the world. Hepatocellular carcinoma (HCC) is a malignancy with high incidence and mortality rates and can lead to death. Cyclooxygenase (COX)-2 is responsible for the development of various cancers, including HCC. Therefore, the use of COX-2 inhibitors can help in the prevention and treatment of cancer. Objectives: The present study aimed to synthesize and examine the effect of imidazolium [1,2-a] piperidinium (4cl-A) and benzo [d] imidazo [1,2-b] thiazolium (1-naphtyl-C) compounds as COX-2 inhibitors on the rat model of HCC. Methods: Animals were randomly assigned to control and HCC induction groups. The study duration was 15 weeks. The HCC was induced using DEN (200 mg/kg, ip) at a single dose and 2-AAF (dietary, 0.02% w/w, for 2 weeks). After 15 weeks, the investigation focused on mitochondrial toxicity parameters. One-way and two-way ANOVA statistical tests were used to analyze the data. Results: The results showed that 4cl-A and 1-naphtyl-C can reduce mitochondrial activity, increase the level of free radicals (ROS), collapse in mitochondrial membrane potential (MMP), cause swelling of mitochondria, and release cytochrome c from HCC mitochondria. While this effect was not observed in healthy mitochondria. Conclusions: The results of the study indicate that these COX-2 inhibitors, along with selected drugs, can help in the treatment of HCC. However, more clinical studies should be conducted. | en |
| DOI | https://doi.org/10.5812/ijpr-164947 | en |
| Keyword | Hepatocellular Carcinoma | en |
| Keyword | COX-2 Inhibitors | en |
| Keyword | Mitochondria | en |
| Keyword | Animal Model | en |
| Publisher | Brieflands | en |
| Title | Evaluation of Anticarcinogenic and Cytotoxic Effects of COX-2 Specific Inhibitors on an Animal Model of Hepatocellular Carcinoma Using Isolated Mitochondria | en |
| Type | Research Article | en |