Characterization of AmpC, CTX-M and MBLs types of β-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli producing Extended Spectrum β-lactamases in Kerman, Iran
| Author | Shahla Mansouri | en |
| Author | Davood Kalantar Neyestanaki | en |
| Author | Mostafa Shokoohi | en |
| Author | Shahnaz Halimi | en |
| Author | Reza Beigverdi | en |
| Author | Fereshteh Rezagholezadeh | en |
| Author | Ali Hashemi | en |
| Issued Date | 2014-02-01 | en |
| Abstract | Background:: Extended spectrum β-lactamases (ESBLs) and AmpC β-lactamases enzyme are major sources of resistance to β-lactam antibiotics especially in Enterobacteriaceae such as Escherichia coli and Klebsiella pneumoniae. Increasing frequency of the co-existence of ESBLs with AmpC-β-lactamases in bacteria is a serious threat for treating bacterial infections. Objectives:: The aim of this study was to determine the presence of AmpC and CTX-M types of β-lactamases in clinical isolates of E. coli and K. pneumoniae producing ESBLs. Materials and Methods:: Resistance to different antibiotics was determined using the standard disk diffusion method. ESBLs, MBLs and AmpC-β-lactamases were detected by the combination double disk test (CDDT) method and polymerase chain reaction (PCR) was used to determine blaCTX-M genes in the ESBLs and AmpC positive isolates. Results:: The prevalence of ESBLs and AmpC-β-lactamase producer isolates was 181 (43.8%) and 133 (37.2%), respectively. The prevalence of blaCTX-M among isolates was 61 (14.7%). Conclusions:: Outbreak of isolates co-expressing AmpC-β-lactamases and ESBLs can cause serious problems in the future, regarding the treatment of infections caused by these common enteric pathogens. | en |
| DOI | https://doi.org/10.5812/jjm.8756 | en |
| Keyword | Antibiotic Resistance | en |
| Keyword | ESBLs | en |
| Keyword | AmpC-β-lactamases | en |
| Keyword | <i>bla</i><sub><i>CTX</i><i>-M</i></sub> | en |
| Keyword | <i>Escherichia coli</i> | en |
| Keyword | <i>Klebsiella pneumoniae</i> | en |
| Publisher | Brieflands | en |
| Title | Characterization of AmpC, CTX-M and MBLs types of β-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli producing Extended Spectrum β-lactamases in Kerman, Iran | en |
| Type | Research Article | en |
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