A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran
| Author | Hamid Abdollahi | en |
| Author | Mohammad Javad Zahedi | en |
| Author | Sodief Darvish Moghadam | en |
| Author | Mehdi Hayatbakhsh Abasi | en |
| Issued Date | 2011-04-30 | en |
| Abstract | Introduction and objective: Metronidazole (Mtz) resistance in Helicobacter pylori has been found to be associated with mutations in rdxA, a gene encoding an oxygen insensitive NADPH nitroreductase, and enhanced by mutations in frxA a gene encoding a NAD(P)H-flavin oxidoreductase. In this study, we examined the prevalence of rdxA deletion among local isolates of H. pylori.Materials and methods: We tested 63 H. pylori isolates obtained from 191 patients referred to endoscopy unit of Afzalipour hospital in Kerman, Iran, during 2009 for their susceptibility to common anti-H. pylori antibiotics using a modified disk diffusion test. Then the metronidazole resistant and sensitive isolates were evaluated for deletion in rdxA gene by PCR methods and compared with each other.Results: From 35 resistant H. pylori isolates in this study only 22.9% (8 isolates) detected with deletion rdxA gene. No rdxA deletion was detected in sensitive isolates.Conclusion: According to rdxA deletion rates in this study, it seems that some other nitroreductases are involved in metronidazole activation or there are other metronidazole resistance mechanisms involved. | en |
| DOI | https://doi.org/ | en |
| Keyword | Helicobacter pylori | en |
| Keyword | Metronidazole resistant | en |
| Keyword | rdxA deletion | en |
| Publisher | Brieflands | en |
| Title | A study of rdxA gene deletion in metronidazole resistant and sensitive Helicobacter pylori isolates in Kerman, Iran | en |
| Type | Research Article | en |
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