The VDR and TNF-? Gene Polymorphisms in Iranian Tuberculosis Patients: The Study on Host Susceptibility
| Author | Mohammed Varahram | en |
| Author | Parissa Farnia | en |
| Author | Sabar Anoosheh | en |
| Author | Mehdi Kazampour | en |
| Author | Muayad Merza | en |
| Author | Shima Saeif | en |
| Author | Mohammad Reza Masjedi | en |
| Author | Ali Akbar Velayati | en |
| Orcid | Parissa Farnia [0000-0003-3676-2018] | en |
| Issued Date | 2009-10-31 | en |
| Abstract | Background: VitaminD receptor (VDR) and tumor necrosis factoralpha (TNF-?) genes are thought to be important in the intracellular killing of mycobacteria. This study aimed to determine the association of VDR and TNF-? variant with development of pulmonary tuberculosis (PTB) among Iranian patients. Patients and methods: Selected regions of VDR and TNF-? were amplified, and then the PCR products were digested using restriction enzyme (RFLP). Digested products were run on 8% polyacrylamide gel, and were stained with silver nitrate. Single nucleotide polymorphisms (SNPs) at restriction sites of BsmI, and FokI of VDR gene and SNPs of TNF-? at -238,-308, -244,-857,-863 positions were analyzed by PCR-RFLP among 117 PTB cases and 60 healthy controls. Results: No statistically significant difference was observed in allele frequencies of FokI of VDR and TNF-? at -238, -244,-863 and 857 position. Although, the frequency of b allele of BsmI (p=0.001) and -308 A variant in TNF-? promoter region (p=0.006) were significantly more in PTB patients than healthy controls. The frequency of extended diplotypes were different in patients and control subjects (p Conclusion: This study confirmed the association of VDR BsmI and TNF-? -308A with susceptibility to tuberculosis in Iranian PTB patients. In addition, the results showed the importance of haplotypes and diplotypes analysis in determining the host susceptibility against TB | en |
| DOI | https://doi.org/ | en |
| Keyword | VDR | en |
| Keyword | TNF-? gene | en |
| Keyword | Tuberculosis. | en |
| Publisher | Brieflands | en |
| Title | The VDR and TNF-? Gene Polymorphisms in Iranian Tuberculosis Patients: The Study on Host Susceptibility | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 73376-pdf.pdf
- Size:
- 191.48 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF