Estimation of Group B Streptococcus Colonization in High-Risk Neonates by PCR and Standard Culture
| Author | Fariba Shirvani | en |
| Author | Nader Shahrochi | en |
| Author | Mitra Radfar | en |
| Author | Davood Lakestani | en |
| Orcid | Fariba Shirvani [0000-0002-4893-6452] | en |
| Issued Date | 2014-10-01 | en |
| Abstract | Background:: Group B Streptococcus (GBS) (Streptococcus agalactiae) is the leading cause of morbidity and mortality of newborn infants considered a leading factor causing septicemia after birth. The standard method for the diagnosis of GBS colonization is culture in a selective medium, but PCR has a high sensitivity and specificity. Objectives:: The goal of this study was to estimate the colonization of GBS in rectum of neonates of high-risk mothers by culture and PCR method. Patients and Methods:: Samples were taken from rectal mucosa of 154 neonatesof high-risk mothers for GBS by swabs. Samples were tested by standard culture using Todd Hewitt broth and blood agar and also by PCR using primers specific for cfb gene. Results:: Of 154 neonates, Culture identified 17 (11%) neonates as colonized by GBS; and the PCR assay could identify 27 (17%) neonates with positive results for GBS. Mothers age range was 17-40 years (mean = 26.1 ± 5.1). Maternal age was significantly lower in PCR positive group (P = 0.038) and in culture positive group (P = 0.015). Using culture as the gold standard, sensitivity, NPV, specificity, and PPV of PCR were 100%, 100%, 92%, and 62%, respectively. The time required for PCR assay and culture were 2hours and 36hours, respectively. Conclusions:: This study showed that the incidence of GBS in Iranian high-risk neonates is high, so we strongly recommend screening of high-risk neonates for detection of GBS. | en |
| DOI | https://doi.org/10.5812/pedinfect.17345 | en |
| URI | https://brieflands.com/journals/apid/articles/20231 | en |
| Keyword | <i>Streptococcus agalactiae</i> | en |
| Keyword | Infant | en |
| Keyword | Newborn | en |
| Keyword | Colonization | en |
| Publisher | Brieflands | en |
| Title | Estimation of Group B Streptococcus Colonization in High-Risk Neonates by PCR and Standard Culture | en |
| Type | Research Article | en |
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