GABA and Astaxanthin Improve Markers of Streptozotocin-Induced Type 1 Diabetes in Rats: Additive Effects of Combination Therapy

AuthorAlireza Lotfien
AuthorMohammad Rasool Khazaeien
AuthorSajad Fakhrien
AuthorElham Ghanbarien
AuthorMaryam Bozorgien
AuthorMozafar Khazaeien
OrcidAlireza Lotfi [0000-0003-2158-6082]en
OrcidMohammad Rasool Khazaei [0000-0001-9818-0625]en
OrcidSajad Fakhri [0000-0001-8265-8284]en
OrcidElham Ghanbari [0009-0009-7791-4242]en
OrcidMozafar Khazaei [0000-0003-0536-3217]en
Issued Date2026-02-28en
AbstractBackground: Type 1 diabetes (T1D) is an autoimmune disease primarily caused by inflammation and oxidative stress. The treatment of this condition has encountered numerous challenges and limitations to date. Astaxanthin (AST) is a carotenoid known for its antioxidant properties. Gamma-aminobutyric acid (GABA) is a vital neurotransmitter that regulates the activity of beta cells in pancreatic islets. Objectives: The present study aimed to investigate the effects of AST and GABA alone and in combination on beta cell regeneration, fasting blood sugar (FBS), and oxidative stress in streptozotocin (STZ)-induced T1D in rats. Methods: In this experimental in vivo study, 30 male Wistar rats were used. Diabetes was induced with intraperitoneal injection of STZ (55 mg/kg). We randomly divided the rats into five groups (n = 6): control, diabetic, AST (20 mg/kg), GABA (100 mg/kg), and combination therapy (GABA + AST). Following 21 days of treatment, we measured serum FBS, C-peptide, insulin, nitric oxide (NO), and total antioxidant capacity (TAC). We removed the rats’ pancreases to examine gene expression (PDX1, NEUROG3) and histological changes. Results: The FBS and NO levels of treated diabetic rats with GABA, AST, and their combination decreased significantly (P < 0.05). The amount of C-peptide, insulin, TAC, and expression of PDX1 and NEUROG3 genes increased significantly. Conclusions: AST and GABA reduce FBS and improve serum and histology factors of the pancreas in diabetic rats.en
DOIhttps://doi.org/10.5812/jjnpp-161869en
URIhttps://brieflands.com/journals/jjnpp/articles/161869en
Keyword Diabetes Mellitus Type 1en
KeywordGamma-Aminobutyric Aciden
KeywordAstaxanthinen
KeywordBeta Cellsen
KeywordHyperglycemiaen
PublisherBrieflandsen
TitleGABA and Astaxanthin Improve Markers of Streptozotocin-Induced Type 1 Diabetes in Rats: Additive Effects of Combination Therapyen
TypeResearch Articleen

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