Bioassay Guided Fractionation of an Anti-Methicillin-Resistant Staphylococcus aureus Flavonoid From Bromus inermis Leyss Inflorescences
| Author | Atousa Aliahmadi | en |
| Author | Fateme Mirzajani | en |
| Author | Alireza Ghassempour | en |
| Author | Ali Sonboli | en |
| Issued Date | 2014-12-01 | en |
| Abstract | Background: Plants are considered as promising sources of new antibacterial agents as well as bioassay guided fractionation. Objectives: In the present work, the antibacterial properties, especially against methicillin-resistant Staphylococcus aureus (MRSA), of Bromus inermis inflorescence was studied, using the bioassay guided fractionation as well as the bio-autographic method. Materials and Methods: The plant organic extract was prepared via maceration in methanol, followed by the fractionation using n-hexane. The extracts were subjected for minimum inhibitory concentrations (MICs) against some human pathogenic bacteria via standard broth micro-dilution assay. Thereafter, a bio-autographical method was applied using the high performance thin layer chromatography (HPTLC) coupled with agar overlay assays for the primary characterization and identification of bioactive substance (s). Results: Through the bioassay guided fractionation method, the greatest antibacterial activities were related to the n-hexane extract. It was also revealed that the effective anti-MRSA agent of the assessed plant was a relatively polar substance with an MIC value of about 8 μg/mL against the tested MRSA strain (in comparison with the MIC value of 32 μg/mL for chloramphenicol). Conclusions: As a result of the full range UV-Vis scanning of the responsible band in the HPTLC experiments (200-700 nm), the flavonoid was the most imaginable natural compound. | en |
| DOI | https://doi.org/10.5812/jjm.12739 | en |
| Keyword | Chemical Fractionation | en |
| Keyword | Methicillin-Resistant <i>Staphylococcus aureus</i> | en |
| Keyword | Flavonoid | en |
| Keyword | Anti-bacterial Agents | en |
| Publisher | Brieflands | en |
| Title | Bioassay Guided Fractionation of an Anti-Methicillin-Resistant Staphylococcus aureus Flavonoid From Bromus inermis Leyss Inflorescences | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 18788-pdf.pdf
- Size:
- 380.8 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF