Optimization of Calcium Alginate Hydrogel Bioencapsulation of <i>Acinetobacter junii</i> B6, a Lipopeptide Biosurfactant Producer
| Author | Mohammadhossein Ahmadi Borhanabadi | en |
| Author | Mohammad Amin Raeisi Estabragh | en |
| Author | Gholamreza Dehghannoudeh | en |
| Author | Ibrahim M Banat | en |
| Author | Mandana Ohadi | en |
| Author | Mohammad Hassan Moshafi | en |
| Orcid | Mohammad Amin Raeisi Estabragh [0000-0001-5992-1654] | en |
| Issued Date | 2023-05-31 | en |
| Abstract | Background: Biosurfactants are derived from microbes, plants, and animals. Acinetobacter junii B6 is a lipopeptide biosurfactant producer previously investigated for its structure, physicochemical, and product aggregation properties. Objectives: In this study, we investigated and optimized the bioencapsulation of A. junii B6 in calcium alginate hydrogel. Methods: Acinetobacter junii B6 was encapsulated using calcium alginate hydrogel. The formulation of the hydrogel was optimized using a full factorial approach. Sodium alginate concentration, calcium chloride concentration, and hardening time were selected as the main factors, and surface tension was the response measure. A scanning electron microscope (SEM) was used to study the bead's morphology. Results: Scanning electron microscope image showed rounded and smooth beads. The most biosurfactant production and reduced surface tension (35.98 mN/m) were observed at concentrations of 1% calcium chloride, (1%) sodium alginate, and 15 minutes of hardening time. Acinetobacter junii B6 can be encapsulated in alginate hydrogels producing biosurfactant at optimum experimental design. Conclusions: This represents a practical method for optimizing the bioencapsulation of A. junii B6 to produce biosurfactants. | en |
| DOI | https://doi.org/10.5812/jjnpp-134325 | en |
| Keyword | <i>Acinetobacter junii</i> B6 | en |
| Keyword | Bioencapsulation | en |
| Keyword | Biosurfactant | en |
| Keyword | Calcium Alginate | en |
| Keyword | Optimization | en |
| Publisher | Brieflands | en |
| Title | Optimization of Calcium Alginate Hydrogel Bioencapsulation of <i>Acinetobacter junii</i> B6, a Lipopeptide Biosurfactant Producer | en |
| Type | Research Article | en |