Doxorubicin Loaded DNA Aptamer Linked Myristilated Chitosan Nanogel for Targeted Drug Delivery to Prostate Cancer
| Author | Fereshteh Atabi | en |
| Author | Seyed Latif Mousavi Gargari | en |
| Author | Mehrdad Hashemi | en |
| Author | Parichehreh Yaghmaei | en |
| Issued Date | 2017-01-31 | en |
| Abstract | Recently, specific attention has been paid to aptamers, short DNA or RNA, as a tool for cancer diagnosis and therapy. In the present study MCS nanogels were prepared by Myristate: Chitosan at 1:9 ratio and were characterized by several techniques. A selected ssDNA aptamer (Apt) capable of detecting LNCaP cells was linked to Myristilated Chitosan nanogels (Apt-MCS) by glutaraldehyde and loaded with Doxorubicin (DOX) to be used in targeted drug delivery against the Prostate cancer cells. LNCaP and PC-3 cells were treated with Apt-MCS-DOX complex and the binding efficiency was estimated by flow cytometry. The binding affinity of the selected aptamers was above 70% compared to the initial library. The loading capacity of the nanogel was as high as 97% and up to 40% of DOX were released from MCS within 15 days. Cytotoxicity of nanodrug on LNCaP cells was determined by MTT assay. Apt-MCS-DOX was specifically binded to LNCaP cells whereas it didn’t show any specificity to PC-3 cells as a negative control. Both MCS-DOX and Apt-MCS-DOX showed a lethal effect on LNCaP cells. Our results can lead to an aptamer based simple and applicable technique for early diagnosis and treatment of cancerous cells. | en |
| DOI | https://doi.org/10.22037/ijpr.2017.1948 | en |
| Keyword | DOX | en |
| Keyword | MCS | en |
| Keyword | Nanogel | en |
| Keyword | Aptamer | en |
| Keyword | Targeted drug delivery | en |
| Keyword | Prostate cancer | en |
| Publisher | Brieflands | en |
| Title | Doxorubicin Loaded DNA Aptamer Linked Myristilated Chitosan Nanogel for Targeted Drug Delivery to Prostate Cancer | en |
| Type | Original Article | en |