In Silico B Cell and T Cell Epitopes Evaluation of lipL32 and OmpL1 Proteins for Designing a Recombinant Multi-Epitope Vaccine Against Leptospirosis
| Author | Narges Nazifi | en |
| Author | Seyyed Mojtaba Mousavi | en |
| Author | Saeedeh Moradi | en |
| Author | Amin Jaydari | en |
| Author | Mohammad Hassan Jahandar | en |
| Author | Ali Forouharmehr | en |
| Issued Date | 2018-04-30 | en |
| Abstract | Leptospirosis is a widespread zoonotic disease caused by Leptospira interrogans. The conventional vaccines have some major problems. Therefore, recombinant vaccines such as multiple-epitope vaccine are suggested. OmpL1 and lipL32 are the most important proteins of Leptospira interrogans bacteria that can be used in epitope prediction process to design a multiple-epitope vaccine. Hence, in this study, the most reliable and accurate online servers were applied to predict B cell and T cell epitopes, the secondary and tertiary structures, enzyme digestion, and antigenicity score of ompL1 and lipL32. The results showed that epitopes located at 103 - 122, 210 - 232, and 272 - 291 amino acid residues are the common epitopes between T cell (MHCI) and B cell. 288 - 308 amino acid residues were introduced as common epitopes to stimulate both T cell (MHCI and MHCII) and B cell of ompL1 protein. In the case of LipL32 protein, 80 - 96 amino acid residues are recommended for T cell epitopes and 63-81 amino acid residues for stimulation of both B and T cells. All the mentioned epitopes can be considered as linear epitopes in designing a recombinant vaccine based on chimeric epitopes. It appears that these epitopes can be applied to design recombinant multiple-epitope vaccines against leptospirosis. | en |
| DOI | https://doi.org/10.5812/iji.63255 | en |
| Keyword | Epitope | en |
| Keyword | Leptospira | en |
| Keyword | Vaccine | en |
| Keyword | Zoonosis | en |
| Publisher | Brieflands | en |
| Title | In Silico B Cell and T Cell Epitopes Evaluation of lipL32 and OmpL1 Proteins for Designing a Recombinant Multi-Epitope Vaccine Against Leptospirosis | en |
| Type | Research Article | en |