Characterization of CTX-M-Type Extend-Spectrum β-Lactamase Producing <i>Klebsiella </i>spp. in Kashan, Iran
| Author | Hasan Afzali | en |
| Author | Farzaneh Firoozeh | en |
| Author | Atena Amiri | en |
| Author | Rezvan Moniri | en |
| Author | Mohammad Zibaei | en |
| Issued Date | 2015-10-31 | en |
| Abstract | Context:: The CTX-M family consists of more than 50 β-lactamases, which are grouped on the basis of sequences into five subtypes including CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9 and CTX-M-25. Objectives:: The current study aimed to detect subtypes of CTX-M extended-spectrum β-lactamases (ESBLs) among ESBL positive Klebsiella isolates from patients in Kashan, Iran. Materials and Methods:: A total of 100 clinical isolates of Klebsiella were collected and the isolates, which showed resistance or reduced susceptibility to cefotaxime, ceftazidime and/or aztreonam by the disk diffusion method were selected. These isolates were identified as ESBL-producing isolates by double disk synergy tests using clavulanic acid, cefotaxime, ceftazidime and aztreonam. The blaCTX-M type determinants were identified by the Polymerase Chain Reaction (PCR) method followed by DNA sequencing. Results:: Of the 100 Klebsiella isolates, 41 (41%) demonstrated resistance or reduced susceptibility to ceftazidime and/or aztreonam and 35% (n = 35) were ESBL-producers. Twenty-eight (8o%) of the ESBL-producing isolates carried the blaCTX-M type genes. Based on PCR assays and sequencing of blaCTX-M genes, CTX-M-1, CTX-M-2 and CTX-M-9 were identified in 21 (60%), 15 (42%) and nine (34%) of these isolates, respectively (GenBank accession numbers KJ803828-KJ803829). Conclusions:: Our study showed that the frequency of blaCTX-M genes among Klebsiella isolates in our region is at an alarming rate. Also, we found a high prevalence of blaCTX-M-1 β-lactamase in Klebsiella isolates in Kashan. | en |
| DOI | https://doi.org/10.5812/jjm.27967 | en |
| Keyword | Antibiotic Resistance | en |
| Keyword | Blactx-M | en |
| Keyword | Clinical Specimens | en |
| Keyword | ESBLs | en |
| Keyword | <i>Klebsiella </i>spp. | en |
| Publisher | Brieflands | en |
| Title | Characterization of CTX-M-Type Extend-Spectrum β-Lactamase Producing <i>Klebsiella </i>spp. in Kashan, Iran | en |
| Type | Research Article | en |
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