Expression of MicroRNA-155 is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B Patients
| Author | Su-Lin Yu | en |
| Author | Hong Deng | en |
| Author | Xin-Hua Li | en |
| Author | Ya-Xin Huang | en |
| Author | Dong-Ying Xie | en |
| Author | Zhi-Liang Gao | en |
| Issued Date | 2016-01-01 | en |
| Abstract | Background: Persistent hepatitis B virus (HBV) infection is sustained by inadequate immune responses, either natural or acquired. Recent studies have suggested that immune responses to viral infection may be affected by microRNA (miR)-155, via its involvement in immune cell differentiation and maturation. However, little is known on the specific interaction between miR-155 and HBV in host antiviral immunity. Objectives: This study evaluated the levels of miR-155 in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients, relative to that of healthy subjects, and investigated an association between miR-155 levels and HBV DNA or alanine aminotransferase (ALT). Patients and Methods: Total RNA was extracted from peripheral venous blood samples of 90 treatment-naive patients with chronic HBV infection and 20 healthy volunteers. The levels of miR-155 in the PBMCs were measured by real-time quantitative polymerase chain reaction. Serum HBV DNA and liver enzymes were estimated using standard clinical laboratory methods. Results: In the HBV-infected patients, the miR-155 levels were significantly lower than in the healthy controls (P = 0.001). Chronic HBV-infected patients with elevated ALT had higher levels of miR-155 compared with patients with normal ALT (P = 0.014). No correlations were found between miR-155 and ALT or HBV DNA. Conclusions: The miR-155 appeared to be suppressed during HBV infection. The significantly higher miR-155 levels in ALT-elevated patients infected with HBV suggest that miR-155 levels in PBMCs correlate with the immune state of patients with chronic HBV infection. | en |
| DOI | https://doi.org/10.5812/hepatmon.34483 | en |
| Keyword | Hepatitis B | en |
| Keyword | Chronic | en |
| Keyword | Immunity | en |
| Keyword | Alanine Transaminase | en |
| Keyword | Leukocytes | en |
| Keyword | Mononuclear | en |
| Keyword | miR-155 | en |
| Publisher | Brieflands | en |
| Title | Expression of MicroRNA-155 is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B Patients | en |
| Type | Research Article | en |
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