Visceral Fat ABCG1, ABCG5 and Visfatin Gene Expression in Response to a Treadmill Running Program with or without a Liquid Pistachioatlantica (Bene) Extraction in Female Rats

Abstract

ATP-binding cassette (ABC) transporters including ABCG1 and ABCG5, use the energy of ATP hydrolysis to translocate a wide variety of substrates across biological membranes. Visfatin, a novel adipokine, was revealed to be associated with obesity and to have insulin mimetic effect that is highly expressed in visceral adipose tissue. The aim of this study was to determine the visceral fat ABCG1, ABCG5 and visfatin relative gene expression. Twenty wistar rats (6-8 weeks old and 125-135 g weight) were used. Animals were randomly assigned into saline-control (SC), saline-training (ST), and Bene-control (BC), and Bene-training (BT). Training groups was given exercise on a motor-driven treadmill at 25 m/min (0% grade) for 60 min/day and 5 days/week for eight weeks. Subjects were fed oral, with Bene extraction and saline for four weeks. ABCG1, ABCG5 and visfatin relative genes expression was detected by Real-time PCR method. Results demonstrated that Bene extraction significantly reduces ABCG1 and ABCG5 relative gene expression and increase visfatin relative gene expression in visceral fat. Exercise training significantly reduces visfatin relative gene expression and increases ABCG1 and ABCG5 relative gene expression in visceral fat