Determination of Cytomegalovirus Prevalence and Glycoprotein B Genotypes Among Ulcerative Colitis Patients in Ahvaz, Iran
| Author | Reza Taherkhani | en |
| Author | Fatemeh Farshadpour | en |
| Author | Manoochehr Makvandi | en |
| Author | Mojtaba Hamidifard | en |
| Author | Mahdi Esmailizadeh | en |
| Author | Bijan Ahmadi | en |
| Author | Hamid Heidari | en |
| Orcid | Manoochehr Makvandi [0000-0003-2510-3033] | en |
| Issued Date | 2015-02-28 | en |
| Abstract | Background:: The human cytomegalovirus (HCMV) is a common pathogen which usually remains asymptomatic in the healthy adults; however, it can cause a symptomatic disease in the immunocompromised patients. The risk of infection with HCMV increases in ulcerative colitis (UC) patients as a result of receiving immunosuppressive agents. Objectives:: This study aimed to determine the prevalence and the glycoprotein B genotypes of HCMV among the patients with HCMV disease superimposed on an UC flare that required hospitalization in Imam Khomeini Hospital in Ahvaz, Iran, during 2010- 2012. Patients and Methods:: In this case-control study, formalin-fixed paraffin-embedded intestinal tissue samples were taken from 98 patients with UC disease including 53 males and 45 females (mean age ± standard deviation, 38.95 ± 17.93) and 67 control patients with noninflammatory disease who were referred to Imam Khomeini Hospital during 2010-2012. Detection of HCMV genome in intestinal samples was carried out by seminested polymerase chain reaction. Glycoprotein B genotypes were determined by sequencing. Results:: Among 98 patients with UC, only 12 (12.2%) patients were positive for HCMV genome, while the HCMV genome was not detected in any of the controls. (P = 0.002). The distribution of HCMV gB genotypes in 12 CMV-positive UC patients was as follow: gB1, 11 (91.7%) and gB3, 1 (8.3%). The most prevalent genotype in CMV-positive UC patients was gB1. Conclusions:: In this study, high prevalence of 91.7% HCMV gB1 genotype was predominant among HCMV-positive UC patients, which suggests that there might be an association between HCMV gB genotype 1 and UC disease. | en |
| DOI | https://doi.org/10.5812/jjm.17458 | en |
| Keyword | Human Cytomegalovirus | en |
| Keyword | Ulcerative Colitis | en |
| Keyword | gB | en |
| Keyword | Genotypes | en |
| Keyword | Nested PCR | en |
| Keyword | Intestinal | en |
| Keyword | Tissue | en |
| Publisher | Brieflands | en |
| Title | Determination of Cytomegalovirus Prevalence and Glycoprotein B Genotypes Among Ulcerative Colitis Patients in Ahvaz, Iran | en |
| Type | Research Article | en |
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