Loop-mediated Isothermal Amplification Test for Rapid Identification of Clinical <i>Nocardia</i> Isolates
| Author | Mojtaba Taghizadeh Armaki | en |
| Author | Mohammad Hossein Tayefeh-Arbab | en |
| Author | Jalal Jafarzadeh | en |
| Author | Sina Nasrollahian | en |
| Author | Zohre Baseri | en |
| Author | Hamed Mehdinezhad | en |
| Author | Ramazan Rajabnia | en |
| Author | Abazar Pournajaf | en |
| Orcid | Mojtaba Taghizadeh Armaki [0000-0003-1811-5829] | en |
| Orcid | Jalal Jafarzadeh [0000-0002-5626-5150] | en |
| Orcid | Abazar Pournajaf [0000-0002-3407-0950] | en |
| Issued Date | 2022-12-31 | en |
| Abstract | Background: Nocardia is a Gram-positive and partially acid-fast bacterium. The species are widely distributed in the environment and cause severe human infections. Nocardiosis is not easily identifiable due to the lack of pathognomonic clinical signs. Objectives: The present study was designed to develop and evaluate a simple and quick method based on a loop-mediated isothermal amplification (LAMP) assay for detecting Nocardia spp isolated from bronchoalveolar lavage (BAL) samples. Methods: In this cross-sectional study, 357 BAL samples were collected from two teaching hospitals. The polymerase chain reaction (PCR) was performed using a set of species-specific primers for the 16S rRNA gene. Kinyoun acid-fast staining and culture were done on the Sabouraud dextrose plate. The optimal LAMP reaction condition was set at 65°C for 45 min, with the recognition limit as 1 pg DNA/tube and 100 CFU/reaction. In addition to calcein and manganous ions, agarose gel electrophoresis was used to visualize the amplified LAMP products. Results: Out of 357 BAL samples, 0 (0.0%), 4 (1.1%), 9 (2.5%), and 10 (2.8%) Nocardia strains were identified by direct staining of partial acid-fast, streak culture plate, PCR, and LAMP methods, respectively. Conclusions: We developed a new LAMP technique for the recognition of Nocardia, which is fast, very precise, simple, and low-cost. According to our knowledge, this is the first report of the LAMP method to detect Nocardia in clinical samples. | en |
| DOI | https://doi.org/10.5812/jjm-132432 | en |
| URI | https://brieflands.com/journals/jjm/articles/132432 | en |
| Keyword | Loop-mediated Isothermal Amplification Test | en |
| Keyword | <i>Nocardia</i> | en |
| Keyword | Clinical Sample | en |
| Keyword | Rapid Detection | en |
| Publisher | Brieflands | en |
| Title | Loop-mediated Isothermal Amplification Test for Rapid Identification of Clinical <i>Nocardia</i> Isolates | en |
| Type | Research Article | en |
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