Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma
| Author | Seyed Mohsen Foroutan | en |
| Author | Afshin Zarghi | en |
| Author | Alireza Shafaati | en |
| Author | Arash Khoddam | en |
| Issued Date | 2006-01-31 | en |
| Abstract | A simple, rapid and sensitive high-performance liquid chromatographic (HPLC) method for the determination of risperidone in human plasma was developed. An HPLC system based on a Nucleosil C8 column (150×4 mm) and a UV detector (λ= 280 nm) were used. A mixture of sodium dihydrogen phosphate buffer-acetonitrile (55:45, v/v) adjusted to pH 6.0 at a flow rate of 1.5 ml min-1 was used as mobile phase. The proteins were precipitated with an acetonitrile solution containing diltiazem as internal standard and the average recovery was 93.9±3.4%. The detection limit for risperidone in plasma was 0.5 ngml-1. The calibration curve was linear over the concentration range 2-50 ngml-1. The inter-day and intra-day assay coefficients of variation were found to be less than 5%. The present validated method was successfully used for pharmacokinetic studies of risperidone in human subjects. | en |
| DOI | https://doi.org/10.22037/ijpr.2010.650 | en |
| URI | https://brieflands.com/journals/ijpr/articles/128260 | en |
| Keyword | Risperidone | en |
| Keyword | Plasma | en |
| Keyword | HPLC | en |
| Publisher | Brieflands | en |
| Title | Rapid High Performance Liquid Chromatographic Determination of Risperidone in Human Plasma | en |
| Type | Research Article | en |
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