The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods
| Author | Bardia Farzamfar | en |
| Author | Saeed Bayanolhagh | en |
| Author | Fereidoun Mahboudi | en |
| Author | Mahin Zahrai | en |
| Issued Date | 2007-07-31 | en |
| Abstract | Aflatoxins are a group of fungal toxic metabolites, which are contaminated certain food commodities. ELISA is one of the sensitive methods for detection of aflatoxins. Preparation and stabilizing of a proper conjugated tracer for detection of aflatoxins is probably the main step for designing an ELISA method. In current study, different stabilizers were applied to stabilize a newly prepared conjugated molecule, Horseradish peroxidase-Bovine serum albumin- Aflatoxin B1 (HRP-BSA-AFB1). Stabilizing effects of six different stabilizers were compared during 10 months verification at room temperature and 6 weeks verification at 37°C. Based on the results, it was concluded that trehalose-containing stabilizers especially those contain casein in their compositions show the best stabilizing effects on HRP-BSA-AFB1 conjugated tracer. | en |
| DOI | https://doi.org/10.22037/ijpr.2010.719 | en |
| Keyword | stabilizer | en |
| Keyword | Immunoassay | en |
| Keyword | HRP | en |
| Keyword | Conjugate | en |
| Keyword | BSA | en |
| Keyword | Aflatoxin | en |
| Publisher | Brieflands | en |
| Title | The Effect of Different Stabilizers on Stability of Horseradish Peroxidase- Bovine Serum Albumin-Aflatoxin B1, a Conjugated Tracer for Detection of Aflatoxin B1 in Immunoassay-Based Methods | en |
| Type | Research Article | en |
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