Enhanced RNA Extraction from Stool Samples Using Fe-MSN Nanoparticles: A Comparative Study with Magnetic Beads and Silica Columns
| Author | Hannan Khodaei | en |
| Author | Leila Azimi | en |
| Author | Abbas Akhavan Sepahi | en |
| Author | Fatemeh Ashrafi | en |
| Author | Maryam Rajabnejad | en |
| Orcid | Hannan Khodaei [0000-0002-2297-4733] | en |
| Orcid | Leila Azimi [0000-0002-7216-2530] | en |
| Orcid | Abbas Akhavan Sepahi [0000-0002-4112-8962] | en |
| Orcid | Fatemeh Ashrafi [0000-0003-3966-4990] | en |
| Orcid | Maryam Rajabnejad [0000-0003-3228-2922] | en |
| Issued Date | 2026-01-31 | en |
| Abstract | Background: The human digestive system is known as a diverse collection of microorganisms, which includes viruses. Several viral agents can cause gastroenteritis problems, such as rotavirus, enteroviruses, and coronaviruses like SARS-CoV-2. Reverse transcription polymerase chain reaction (RT-PCR) has been used to identify viral genetic elements in stool samples. An important challenge with stool RT-PCR testing is the difficulty in removing inhibitors present in fecal samples. Objectives: This study aims to systematically compare the efficiency of different RNA extraction methods and assess the advantages of using Fe-doped mesoporous silica nanoparticle column (Fe-MSN) nanoparticles for viral RNA isolation from stool samples. Methods: In this study, three extraction methods were used for collecting RNA: (1) Silica membrane column, (2) Fe-MSN, and (3) automated magnetic bead-based commercial extraction kit. The results were compared by evaluating the cycle threshold (Ct) in multiplex real-time PCR for the SARS-CoV-2 virus gene and RNase P gene. Results: The results demonstrated that Fe-MSN nanoparticles provide a more efficient extraction process, yielding higher RNA purity and lower Ct values in RT-PCR compared to conventional methods. A 5-fold decrease in the Ct value of different studied genes was observed after using the Fe-MSN column for RNA extraction compared to the commercial extraction kit. Conclusions: This research aimed to assess and determine the most dependable and precise technique for isolating total RNA from stool samples. The results of this study showed that the Fe-MSN column is the best choice for this purpose. | en |
| DOI | https://doi.org/10.5812/apid-164719 | en |
| URI | https://brieflands.com/journals/apid/articles/164719 | en |
| Keyword | Mesoporous Silica Nanoparticles | en |
| Keyword | Total RNA | en |
| Keyword | Stool Extraction Kit | en |
| Keyword | Silica Membrane | en |
| Publisher | Brieflands | en |
| Title | Enhanced RNA Extraction from Stool Samples Using Fe-MSN Nanoparticles: A Comparative Study with Magnetic Beads and Silica Columns | en |
| Type | Research Article | en |