Induction of Apoptosis in Human Leukemia Cell Line (HL60) by Animal’s Venom Derived Peptides (ICD-85)
| Author | Abbas Zare Mirakabadi | en |
| Author | Zahra Shahramyar | en |
| Author | Hasan Morovvati | en |
| Author | Mohsen Lotfi | en |
| Author | Ali Nouri | en |
| Issued Date | 2012-07-31 | en |
| Abstract | Our previous studies revealed an inhibitory effect of ICD-85 (Venom derived peptides) on breast cancer cell line MDA-MB231. ICD-85 was also confirmed by in-vivo studies to suppress the breast tumor in mice. However, the exact mechanism of ICD-85 was unknown. Hence, the present study was undertaken to assess the mechanism of ICD-85 effect as an anti-proliferative agent of cancer cells. The effect of ICD-85 on proliferation of HL-60 cancer cells was determined by using the MTT assay. The morphological changes of ICD-85 treated HL-60 cells were observed under transmission electron microscope (TEM). DNA fragmentation analysis was also carried out using gel electrophoresis. ICD-85 induced the marked inhibition of HL60 cell proliferation with an IC50-value of 0.04 μg/mL following 24 h of incubation. ICD-85 treated cells when compared with untreated cells, showed nuclear material condensation, endoplasmic reticulum dilation, mitochondria swelling or degradation, increased cytoplasmic vacuoles, reduction or disappearance in cytoplasmic process and decreased nuclear/cytoplasmic ratio was observed. The characteristic DNA ladder formation of ICD-85-treated cells in agarose gel electrophoresis confirmed the results obtained through the electron microscopy. The results of the present study indicated that ICD-85 inhibited the cancer cell proliferation by inducing cell apoptosis. | en |
| DOI | https://doi.org/10.22037/ijpr.2012.1115 | en |
| Keyword | Anticancer | en |
| Keyword | Apoptosis | en |
| Keyword | HL60 | en |
| Keyword | ICD-85 | en |
| Keyword | Venom | en |
| Keyword | DNA fragmentation | en |
| Keyword | Cytotoxicity | en |
| Publisher | Brieflands | en |
| Title | Induction of Apoptosis in Human Leukemia Cell Line (HL60) by Animal’s Venom Derived Peptides (ICD-85) | en |
| Type | Original Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- ijpr-11-931.pdf
- Size:
- 638.72 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF