Effects of Chrysin-PLGA-PEG Nanoparticles on Proliferation and Gene Expression of miRNAs in Gastric Cancer Cell Line
| Author | Farideh Mohammadian | en |
| Author | Alireza Abhari | en |
| Author | Hassan Dariushnejad | en |
| Author | Alireza Nikanfar | en |
| Author | Younes Pilehvar-Soltanahmadi | en |
| Author | Nosratollah Zarghami | en |
| Issued Date | 2016-08-31 | en |
| Abstract | Background: Recently, Chrysin, as a flavone, has revealed cancer chemo-preventive activity. The present experiment utilized the PLGA-PEG-chrysin complex, and free chrysin, to evaluation of the expression of miR-22, miR-34a and miR-126 in human gastric cell line. Objectives: The purpose of this study was to examine whether nano encapsulating chrysin improves the anti-cancer effect of free chrysin on AGS human gastric cell line. Methods: Properties of the chrysin encapsulated in PLGA-PEG nanoparticles were investigated by SEM, H NMR, and FTIR. The assessment of cytotoxicity on the growth of the human gastric cell line was carried out through MTT assay. After treating the cells with a prearranged amount of pure and encapsulated chrysin, RNA was extracted and the expressions of miR-22, miR-34a and miR-126 were measured by using real-time PCR. Results: With regard to the amount of the chrysin loaded in PLGA-PEG nanoparticles, IC50 value was significantly decreased in nanocapsulatedchrysin, in comparison with free chrysin. This finding has been proved through the further increase of miR-22, miR-34a and miR-126 gene expression of nanocapsulatedchrysin, in comparison with free chrysin. Conclusions: In this study, we revealed that the PLGA-PEG-chrysin is more effective than free chrysin in inhibiting the growth of human gastric cell line. | en |
| DOI | https://doi.org/10.17795/ijcp-4190 | en |
| Keyword | Gastric Cancer | en |
| Keyword | Micro RNA | en |
| Keyword | Chrysin | en |
| Keyword | PLGA-PEG | en |
| Keyword | Real-Time PCR | en |
| Publisher | Brieflands | en |
| Title | Effects of Chrysin-PLGA-PEG Nanoparticles on Proliferation and Gene Expression of miRNAs in Gastric Cancer Cell Line | en |
| Type | Research Article | en |