Effect of Chemotherapeutic Drugs on Caspase-3 Activity, as a Key Biomarker for Apoptosis in Ovarian Tumor Cell Cultured as Monolayer. A Pilot Study
| Author | Ewa L Gregoraszczuk | en |
| Author | Agnieszka Rak-Mardyła | en |
| Author | Janusz Ryś | en |
| Author | Jerzy Jakubowicz | en |
| Author | Krzysztof Urbański | en |
| Issued Date | 2015-10-31 | en |
| Abstract | We aimed to develop a cost-effective and robust method to predict drug resistance in individual patients. Representative tissue fragments were obtained from tumors removed from female patients, aged 24-74 years old. The tumor tissue was taken by a histopathology’s or a surgeon under sterile conditions. Cells obtained by enzymatic dissociation from tumor after surgery, were cultured as a monolayer for 6 days. Paclitaxel, doxorubicin, carboplatin and endoxan alone or in combination were added at the beginning of culture and after 6 days, Alamar blue test was used for showing action on cell proliferation why caspase- 3 activity assays for verifying action on apoptosis. Inhibitory action on cell proliferation was noted in 2 of 12 patients tumor treated with both single and combined drugs. Using caspase-3 assay we showed that 50% of tumor cells was resistant to single chemotherapeutic drugs and 40% for combined. In 2 of 12 tumors, which did not reacted on single drugs, positive synergistic action on cell proliferation was observed in combination of D + E and C + E. This pilot study suggests: 1) monolayer culture of tumor cells, derived from individual patients, before chemotherapy could provide a suitable model for studying resistance for drugs; 2) caspase-3 activity is cheap and useful methods; 3) Alamar blue test should be taken into consideration for measuring cell proliferation. | en |
| DOI | https://doi.org/10.22037/ijpr.2015.1726 | en |
| Keyword | Ovarian cancer | en |
| Keyword | Monolayer cell culture | en |
| Keyword | Chemotherapeutic drugs | en |
| Keyword | Casapase-3 activity | en |
| Keyword | Alamar Blue test | en |
| Publisher | Brieflands | en |
| Title | Effect of Chemotherapeutic Drugs on Caspase-3 Activity, as a Key Biomarker for Apoptosis in Ovarian Tumor Cell Cultured as Monolayer. A Pilot Study | en |
| Type | Original Article | en |