Class A and D Extended-Spectrum β-Lactamases in Imipenem Resistant <i>Pseudomonas aeruginosa</i> Isolated From Burn Patients in Iran
| Author | Sanaz Pakbaten Toupkanlou | en |
| Author | Shahin Najar Peerayeh | en |
| Author | Rahim Pirhajati Mahabadi | en |
| Issued Date | 2015-08-31 | en |
| Abstract | Background:: Pseudomonas aeruginosa remains a leading cause of severe wound infection and mortality in burn patients. Objectives:: The current study aimed to determine the prevalence of Ambler class A and D β-lactamases among P. aeruginosa isolated from infected burn injuries in Tehran, Iran. Patients and Methods:: Bacteriological samples were taken from burn patients with clinical symptoms of burn infection. Fifty Gram-negative, oxidase-positive, catalase- positive bacilli, grown at 42ºC and production of pigment on Mueller-Hinton agar were identified as P. aeruginosa. All of the 50 isolates were examined for antibiotic susceptibility via disk diffusion method, and production of Ambler class A and and D β-lactamases by phenotypic screening test. The presence of Ambler class A and D β-lactamases was confirmed by polymerase chain reaction technique. Results:: The results showed that the majority of isolates (88%) were multi-drug resistant. Out of these 50 imipenem resistant isolates, 7 (14%), 18 (36%), 18 (36%) and 18 (36%) strains were positive for blaPER, blaOXA-10, blaTEM and blaSHV genes alone or in combination, respectively. None of the isolates possessed blaKPC or blaGES genes. Conclusions:: The current study highlights that the high level of resistance to many antibacterial agents and a gradual increase in the degree of PER, OXA-10, SHV and TEM ESBLs among the majority of imipenem resistant P. aeruginosa isolated from patients with burn infection is an enormous threat in burn centers in Iran. | en |
| DOI | https://doi.org/10.5812/jjm.18352v2 | en |
| Keyword | Imipenem | en |
| Keyword | <i>bla</i><sub><i>PER</i></sub> | en |
| Keyword | <i>bla</i><sub><i>OXA-10</i></sub> | en |
| Keyword | <i>bla</i><sub><i>TEM</i></sub> | en |
| Keyword | <i>bla</i><sub><i>SHV</i></sub> | en |
| Keyword | <i>Pseudomonas aeruginosa</i> | en |
| Publisher | Brieflands | en |
| Title | Class A and D Extended-Spectrum β-Lactamases in Imipenem Resistant <i>Pseudomonas aeruginosa</i> Isolated From Burn Patients in Iran | en |
| Type | Research Article | en |
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