The Effect of Ibuprofen on Expression of Cox-1/2-Related miRNAs in MKN- 45 -Derived Cancer Stem-Like Cells
| Author | Hassan Akrami | en |
| Author | Behnam Karimi | en |
| Author | Zohreh Salehi | en |
| Author | Sajjad Sisakhtnezhad | en |
| Issued Date | 2019-06-30 | en |
| Abstract | Context: Ibuprofen is an anti-inflammatory drug that non-selectively blocks cyclooxygenases-1/2 (COX-1/2) enzymes and thus reduces the risk tumorigenesis. This study was designed to detect microRNAs (miRNAs) that target Cox-1/2 mRNA and to investigate the effect of ibuprofen on the expression of the miRNAs in MKN-45-derived gastric cancer stem-like cells (CSLCs). We were also aimed to find signaling pathways modulated by the miRNAs. Subjects and Methods: The miRWalk database was used to recognize miRNAs that targeted Cox-1/2 genes. CSLCs were derived from MKN-45 cell line and were then treated with ibuprofen. Consequently, the effect of ibuprofen was evaluated on the expression of the miRNAs by quantitative real-time polymerase chain reaction (qRT-PCR). Finally, DIANA tools were used to identify signaling pathways that modulated by the miRNAs. Results: Our bioinformatic investigation showed that hsa-mir-16-5p, hsa-mir-483- 5p, and hsa-mir-4669 targeted both Cox-1 and Cox-2 mRNAs. The qRT-PCR results indicated that hsa-mir-16-5p and hsa-mir-4669 were overexpressed 2.34 and 9.47 folds, respectively, while hsamir- 483-5p under-expressed (2.08 folds) in ibuprofen-treated CSLCs relative to untreated cells. Moreover, it found that these miRNAs are involved in PI3K-Akt, P53, transforming growth factorbeta, phosphatidylinositol and insulin signaling pathways, cell cycle, extracellular matrix receptor interaction, gap junction, small cell lung cancer, prostate cancer, and chronic myeloid leukemia. Conclusions: We suggest that ibuprofen may reduce the risk of gastric cancer by affecting the expression of miRNAs that target Cox-1/2. however, further research is necessary to unravel its exact effects. | en |
| DOI | https://doi.org/10.4103/jrptps.jrptps_9_18 | en |
| Keyword | Cancer stem‑like cells | en |
| Keyword | hsa‑mir‑16‑5p | en |
| Keyword | hsa‑mir‑4669 | en |
| Keyword | hsa‑mir‑483‑5p | en |
| Keyword | ibuprofen | en |
| Keyword | microRNA | en |
| Publisher | Brieflands | en |
| Title | The Effect of Ibuprofen on Expression of Cox-1/2-Related miRNAs in MKN- 45 -Derived Cancer Stem-Like Cells | en |
| Type | Research Article | en |