Evaluation of Aβ Deposits in the Hippocampus of a Rat Model of Alzheimer’s Disease After Intravenous Injection of Human Adipose Derived Stem Cells by Immuno- and Thioflavin S-Costaining
| Author | Maryam Doshmanziari | en |
| Author | Arash Sarveazad | en |
| Author | Fatemeh Moradi | en |
| Author | Marjan Shariatpanahi | en |
| Author | Esfandiar Doshmanziari | en |
| Author | Sara Simorgh | en |
| Author | Mina Eftekharzadeh | en |
| Orcid | Marjan Shariatpanahi [0000-0003-3616-9089] | en |
| Issued Date | 2019-01-21 | en |
| Abstract | Background: Alzheimer's disease (AD) is a progressive neuropsychiatric disorder that gradually impairs memory and behavioral functions. Amyloid beta (Aβ) is considered as the most toxic substance in the brain of AD patients. Objectives: The present study was designed to evaluate Aβ deposits by Immuno- and Thioflavin S-costaining in the hippocampus of a rat model of AD after intravenous injection of human adipose-derived stem cells (hADSCs). Methods: Thirty-two male rats were included in the four groups of control, sham, AD and hADSCs. The hADSCs characterization was confirmed by the flow cytometry technique. Immuno- and Thioflavin S-costaining was utilized for detecting Aβ plaques in the hippocampus of a rat model of AD following injection of hADSCs. Results: Statistical analysis revealed that Aβ plaques increased significantly in the AD group compared to the control and sham groups. The administration of hADSCs significantly decreased immunoreactivity and Thio-S-positive plaques in the AD group. We also found that the plaques detected by anti-Aβ antibody (immunohistochemical staining) were significantly more than those distinguished by Thioflavin-S in all the groups. Conclusions: Results showed that hADSCs played an effective role in decreasing amyloids aggregation following migration to the hippocampus of the rat model of AD. | en |
| DOI | https://doi.org/10.5812/thrita.88367 | en |
| Keyword | Alzheimer's Disease | en |
| Keyword | Thioflavin | en |
| Keyword | Amyloid Beta Protein | en |
| Keyword | Stem Cell Transplantation | en |
| Publisher | Brieflands | en |
| Title | Evaluation of Aβ Deposits in the Hippocampus of a Rat Model of Alzheimer’s Disease After Intravenous Injection of Human Adipose Derived Stem Cells by Immuno- and Thioflavin S-Costaining | en |
| Type | Research Article | en |