Assessment of the Effect of Overexpression of Death-Associated Protein Kinases 3 Using PEGFPN1 on Gastric Adenocarcinoma Cell Line (MKN45)
| Author | Parvaneh Movahhed | en |
| Author | Atieh Pourbagheri-Sigaroodi | en |
| Author | Ali Anjam-Najmedini | en |
| Author | Ruhollah Vahabpour | en |
| Author | Faranak Kazerouni | en |
| Orcid | Parvaneh Movahhed [0000-0002-9274-9646] | en |
| Orcid | Faranak Kazerouni [0000-0002-1022-0053] | en |
| Issued Date | 2022-06-30 | en |
| Abstract | Background: Gastric cancer (GC) is one of the most common malignancies worldwide. An in-depth understanding of the molecular mechanisms that underlies tumor GC will lead to breakthroughs in the targeted treatment of GC. Based on multiple lines of evidence, death-associated protein kinase 3 (DAPK3) regulates both programmed cell death including apoptosis and autophagy. The widespread experimental evidence raises the possibility of using DAPK-based gene therapy strategies. Objectives: The aim of this study was to investigate the effect of overexpression of DAPK3 using the PEGFPN1 vector on the gastric adenocarcinoma cell line (MKN45). Methods: The MKN45 cell lines were cultured in a DMEM culture medium and, then, the recombinant vector PEGFPN1-DAPK3 was transfected into the cells by lipofectamine 2000. The effects of the overexpression of the DAPK3 gene on MKN45 cells were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), flow cytometry, and real-time quantitative reverse transcription PCR (qRT-PCR) techniques. Results: Our findings indicated that overexpression of DAPK3 in MKN45 cells not only affects the expression of apoptosis-related genes but also changes the expression of autophagy-related genes. Additionally, overexpression of DAPK3 reduces the metabolic activity of cells. Conclusions: The overexpression of the DAPK3 gene can lead to cell death by both inducing apoptosis and autophagy pathways in the gastric adenocarcinoma cell line (MKN45). This anti-cancer activity may describe a hopeful strategy in the application of novel gene therapy for the treatment of gastric adenocarcinoma; however, further research is required to examine the clinical effectiveness of this strategy in GC treatment. | en |
| DOI | https://doi.org/10.5812/ijcm-118185 | en |
| URI | https://brieflands.com/journals/ijcm/articles/118185 | en |
| Keyword | Neoplasms | en |
| Keyword | Stomach Adenocarcinoma | en |
| Keyword | Autophagy | en |
| Keyword | DAPK3 Protein | en |
| Keyword | Apoptosis | en |
| Publisher | Brieflands | en |
| Title | Assessment of the Effect of Overexpression of Death-Associated Protein Kinases 3 Using PEGFPN1 on Gastric Adenocarcinoma Cell Line (MKN45) | en |
| Type | Research Article | en |