Construction of a DNA Vaccine Encoding Mtb32C and HBHA Genes of Mycobacterium tuberculosis
| Author | Roghayeh Teimourpour | en |
| Author | Ali Sadeghian | en |
| Author | Zahra Meshkat | en |
| Author | Majid Esmaelizad | en |
| Author | Mojtaba Sankian | en |
| Author | Ahmad-Reza Jabbari | en |
| Orcid | Zahra Meshkat [0000-0002-4032-7599] | en |
| Issued Date | 2015-08-01 | en |
| Abstract | Background: Tuberculosis (TB) is a contagious disease caused by Mycobacterium tuberculosis. Development of a new vaccine for tuberculosis requires immunogenic antigens capable of inducing suitable and long-lasting T cell immunity. The emergence of multidrugs and extensively drug-resistant strains of M. tuberculosis has made it a global public health concern. Objectives: DNA vaccine is a straightforward method to introduce antigens to the host. In the present study, two immunodominant mycobacterial antigens (Mtb32C and HBHA) were isolated and cloned into pcDNA3.1 (+) to design and construct a new DNA vaccine. This vector is capable of producing new potent chimeric protein. Materials and Methods: Mtb32C (Rv0125) and heparin-binding haemagglutinin adhesion (HBHA) genes were amplified using polymerase chain reaction (PCR) of M. tuberculosis H37Rv genome and ligated into pcDNA3.1 (+). Colony-PCR and restriction enzyme analysis were used to confirm the accuracy of the cloning procedure. Results: In the current study, recombinant pcDNA3.1 (+) vector containing Mtb32C and HBHA genes was successfully constructed. The desired size of DNA fragment was observed using single and double digestion methods. Conclusions: Mtb32C and HBHA genes were successfully isolated from H37Rv genome and cloned into an eukaryotic expression vector. This vector can be considered as a vaccine to evaluate immune responses in animal models. | en |
| DOI | https://doi.org/10.5812/jjm.21556 | en |
| Keyword | Vaccines | en |
| Keyword | DNA | en |
| Keyword | <i>HBHA</i> protein | en |
| Keyword | <i>Mtb32</i> antigen | en |
| Keyword | <i>Mycobacterium tuberculosis</i> | en |
| Publisher | Brieflands | en |
| Title | Construction of a DNA Vaccine Encoding Mtb32C and HBHA Genes of Mycobacterium tuberculosis | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- 56488-pdf.pdf
- Size:
- 514.42 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF