E-Selectin Gene Polymorphisms in Iranian Chronic Hepatitis B Patients

Abstract

Background and Aims: The aim of this study is to detect the substitutions Ser128Arg (A128C) and Leu554Phe (T554C) which responsible for E-selectin polymorphisms in patients with chronic hepatitis B and healthy controls. We investigated association of the Ser128Arg, Leu554Phe gene polymorphisms in the E-selectin gene as prototypical inflammatory molecules for susceptibility to chronic hepatitis B. Methods: Sixty-three patients with chronic hepatitis B virus infection and 150 healthy subjects were recruited sequentially as they presented to clinic. Genomic DNA was isolated from anti-coagulated peripheral blood Buffy coat using Miller's salting-out method. The presence of the E-selectin gene polymorphisms was determined by using polymerase chain reaction amplification refractory mutation system (ARMS). Results: Distribution of E-selectin 128 (A+C-, A+C+, A-C+) genotypes and E-selectin genotype 554 (C+T-, T+C-, C+T+) genotypes were not statistically significant different in chronic hepatitis B patients and controls by Chi-square test (P=0.41 & 0.96, respectively). Also, Two groups had not statistically significant difference in distribution of frequencies of allele 128 A by Chi-square test (P=0.41), 128 C (P=0.15), allele 554 C (P=0.85), and allele 554 T (P=0.76). Carrying allele 128 A (OR=0.587, 95% CI=0.162-2.124), 128 C (OR=1.526, 95% CI=0.849-2.745), 554 C (OR=1.245, 95% CI=0.128-12.089), and allele 554 T (OR=0.880, 95% CI=0.384-2.014) were not risk factors for susceptibility to chronic hepatitis B infection. Conclusions: Carrying E-selectin gene polymorphisms of Ser128Arg and Leu554Phe are not risk factors for susceptibility to chronic hepatitis B.