Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses
| Author | Mozhdeh Sultani | en |
| Author | Talat Mokhtari Azad | en |
| Author | Mohammadreza Eshragian | en |
| Author | Azadeh Shadab | en |
| Author | Maryam Naseri | en |
| Author | Owrang Eilami | en |
| Author | Jila Yavarian | en |
| Issued Date | 2015-08-31 | en |
| Abstract | Background:: It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently. Objectives:: The objective of this study was recognizing the panel of respiratory RNA viruses by multiplex SYBR Green real-time polymerase chain reaction (PCR). Materials and Methods:: Randomized 172 influenza-negative respiratory specimens of all age groups of hospitalized patients were collected. After RNA extraction, cDNA was synthesized. Three SYBR Green multiplex real-time PCR assays were developed for simultaneous detection of 12 respiratory RNA viruses. Each set of multiplex methods detected four viruses, the first set: respiratory syncytial virus, human metapneumovirus, rhinovirus, enterovirus; the second set: parainfluenza viruses 1 - 4 (PIV1-4); the third set: coronaviruses NL63, 229E, severe acute respiratory syndrome (SARS), and OC43. Results:: Application of the multiplex SYBR Green real-time PCR in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) PIV3, 9 (5.23%) PIV4, and 1 (0.58%) coronavirus NL63. All the positive samples were detected during December to March (2011 - 2012). Conclusions:: Multiplex SYBR Green real-time PCR is a rapid and relatively inexpensive method for detection of respiratory viruses. | en |
| DOI | https://doi.org/10.5812/jjm.19041v2 | en |
| Keyword | SYBR Green | en |
| Keyword | Real-Time PCR | en |
| Keyword | Respiratory Syncytial Viruses | en |
| Publisher | Brieflands | en |
| Title | Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- jjm-08-08-19041.pdf
- Size:
- 455.11 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF