Development of a Sensitive Spectrofluorometric-Multivariate Calibration Method for Enzyme Kinetic of Aldehyde Oxidase
| Author | Mohammad Hossein Sorouraddin | en |
| Author | Ebrahim Fooladi | en |
| Author | Abdolhossein Naseri | en |
| Author | Mohammad-Reza Rashidi | en |
| Issued Date | 2009-07-31 | en |
| Abstract | Attempts to obtain experimental values for the kinetic parameters of phenanthridine oxidation by guinea pig or rabbit liver aldehyde oxidase using common spectrophotometric methods have not been successful due to a lower limit of detection. In the present study, a new spectrofluorimetric assay in combination with a multivariate calibration method for enzymatic kinetic study of aldehyde oxidase activity, using phenanthridine as the substrate, has been developed and validated. | en |
| DOI | https://doi.org/10.22037/ijpr.2010.807 | en |
| Keyword | Phenanthridine | en |
| Keyword | Aldehyde oxidase | en |
| Keyword | Kinetic constant | en |
| Keyword | Partial least square | en |
| Keyword | Spectrofluorometry | en |
| Publisher | Brieflands | en |
| Title | Development of a Sensitive Spectrofluorometric-Multivariate Calibration Method for Enzyme Kinetic of Aldehyde Oxidase | en |
| Type | Research Article | en |
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