Anti-hypercholesterolemic Effect of Berbamine Isolated from <i>Rhizoma Coptidis</i> in Hypercholesterolemic Zebrafish Induced by High-Cholesterol Diet
| Author | Bing Han | en |
| Author | Shuming Kou | en |
| Author | Kai He | en |
| Author | Yulong Han | en |
| Author | Yue Wang | en |
| Author | Tao Huang | en |
| Author | Xia Zhou | en |
| Author | Yubo Xiao | en |
| Author | Xuegang Li | en |
| Author | Xiaoli Ye | en |
| Issued Date | 2018-01-31 | en |
| Abstract | The anti-hypercholesterolemic effect of berbamine (BBM) isolated from Rhizoma Coptidis (RC) was investigated in hypercholesterolemic zebrafish model induced by high-cholesterol (HC) diet. Zebrafish embryo assay revealed no significant difference in morphology and cell death with the treatment of BBM less than 20 μg/mL. In zebrafish larvae, the fluorescently labeled cholesterol in caudal artery was reduced dose-dependently after BBM treatment. For adult zebrafish, administration of 0.2% BBM exhibited a significant decrease in plasma total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-c) levels by 37%, 38% and 28%, respectively, along with a fall in lipid content in liver. Further investigation suggested that the mRNA expression of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) and microsomal triglyceride transfer protein (MTP) in liver were down-regulated and the transcription levels of liver gene low-density lipoprotein receptor (LDLR) and cytochrome P450 polypeptide 1a of subfamily A of family 7 (CYP7A1a) were significantly up-regulated with BBM treatment. Histological study showed that BBM can alleviate hepatic steatosis induced by HC diet. These data suggested that BBM has anti-hypercholesterolemic and hepatoprotective effects. The mechanism probably related to the up-regulation of cholesterol transport and bile acid synthesis as well as inhibition of cholesterol synthesis and lipoprotein assembly or secretion. | en |
| DOI | https://doi.org/10.22037/ijpr.2018.2151 | en |
| Keyword | Berbamine | en |
| Keyword | <i>Rhizoma Coptidis</i> | en |
| Keyword | Zebrafish | en |
| Keyword | Hypercholesterolemia | en |
| Keyword | Vascular lipid accumulation | en |
| Publisher | Brieflands | en |
| Title | Anti-hypercholesterolemic Effect of Berbamine Isolated from <i>Rhizoma Coptidis</i> in Hypercholesterolemic Zebrafish Induced by High-Cholesterol Diet | en |
| Type | Original Article | en |