Oncogenic Function of miR-182-5p Versus Tumor-Suppressive Activities of miR-203, miR-150-5p, and miR-139-5p via Target Gene Regulation in Colon Cancer Metastasis
| Author | Sheryar Afzal | en |
| Author | Ali Attiq | en |
| Orcid | Sheryar Afzal [0000-0001-8387-7550] | en |
| Orcid | Ali Attiq [0000-0001-9430-0255] | en |
| Issued Date | 2025-12-31 | en |
| Abstract | Background: MicroRNAs (miRNAs) play key roles in colorectal cancer (CRC) progression and metastasis. miR-182-5p acts as an oncogenic metastamiR, frequently upregulated in cancers and promoting cell migration and invasion. In contrast, miR-203, miR-150-5p, and miR-139-5p function as tumor suppressors and are often downregulated in CRC. Methods: Expression levels of these four miRNAs were quantified in three CRC cell lines using real-time polymerase chain reaction (RT-PCR). Functional assays, including cell viability, migration, and invasion, were conducted after silencing miR-182-5p or overexpressing the tumor-suppressive miRNAs through mimic transfection. Results: The miR-182-5p was significantly overexpressed and positively correlated with metastatic potential, while miR-203, miR-150-5p, and miR-139-5p were downregulated and inversely associated with metastatic traits. Modulation of these miRNAs reduced CRC cell viability, migration, and invasion. Mechanistically, miR-182-5p enhanced metastasis via ANLN and PDE4D regulation, whereas miR-203, miR-150-5p, and miR-139-5p suppressed metastasis through PDE4D, NEGR1, and ATP11A pathways, respectively. Conclusions: These results highlight the opposing roles of miR-182-5p and the tumor-suppressive miRNAs in CRC metastasis and suggest their potential as biomarkers and therapeutic targets. | en |
| DOI | https://doi.org/10.5812/ijpr-164911 | en |
| URI | https://brieflands.com/journals/ijpr/articles/164911 | en |
| Keyword | Colorectal Cancer | en |
| Keyword | miR-182-5p | en |
| Keyword | Tumor Suppressor microRNAs | en |
| Keyword | Metastasis | en |
| Keyword | Cell Migration and Invasion | en |
| Keyword | Therapeutic Target | en |
| Publisher | Brieflands | en |
| Title | Oncogenic Function of miR-182-5p Versus Tumor-Suppressive Activities of miR-203, miR-150-5p, and miR-139-5p via Target Gene Regulation in Colon Cancer Metastasis | en |
| Type | Research Article | en |