Studying the Stability of S-Layer Protein of <i>Lactobacillus Acidophilus</i> ATCC 4356 in Simulated Gastrointestinal Fluids Using SDS-PAGE and Circular Dichroism
| Author | Neda Eslami | en |
| Author | Rouha Kasra Kermanshahi | en |
| Author | Mohammad Erfan | en |
| Issued Date | 2013-03-31 | en |
| Abstract | Crystalline arrays of proteinaceous subunits forming surface layers (S-layers) are now recognized as one of the most common outermost cell envelope components of prokaryotic organisms. The surface layer protein of Lactobacillus acidophilus ATCC4356 is composed of a single species of protein of apparent molecular weight of 43-46 KDa. Considering the Lactobacillus acidophilus ATCC4356 having the S-layer is stable in harsh gastrointestinal (GI) conditions, a protective role against destructive GI factors which has been proposed for these nanostructures. It opens interesting perspectives in the using and development of this S-layer as a protective coat for oral administration of unstable drug nanocarriers. To achieve this goal, it is necessary to study the in-vitro stability of the S-layers in the simulated gastrointestinal fluids (SGIF). This study was planned to evaluate the in-vitro stability of the extracted S-layer protein of Lactobacillus acidophilus ATCC4356 in SGIF using it as a protective coat in oral drug delivery. Sodium dodecyl sulfate gel electrophoresis (SDS-PAGE) and circular dichroism (CD) spectroscopy were used to study the stability of the S-layer protein incubated in SGIF. Both the SDS-PAGE and CD spectra results showed that Lactobacillus acidophilus ATCC4356 S-layer protein is stable in simulated gastric fluid (SGF) with pH = 2 up to 5 min. It is stable in SGF pH = 3.2 and above it, with and without pepsin. It is also stable in all the simulated intestinal fluids. This S-layer is also stable in all of the simulated intestinal fluids. | en |
| DOI | https://doi.org/10.22037/ijpr.2013.1271 | en |
| Keyword | S-layer protein | en |
| Keyword | <i>Lactobacillus acidophilus </i>ATCC4356 | en |
| Keyword | Stability | en |
| Keyword | Gel electrophoresis | en |
| Keyword | Circular dichroism | en |
| Publisher | Brieflands | en |
| Title | Studying the Stability of S-Layer Protein of <i>Lactobacillus Acidophilus</i> ATCC 4356 in Simulated Gastrointestinal Fluids Using SDS-PAGE and Circular Dichroism | en |
| Type | Original Article | en |
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