Investigation the Effect of Encapsulated Bromelain Enzyme in Magnetic Carbon Nanotubes on Colorectal Cancer Cells
| Author | Ardalan Montazeri | en |
| Author | Mina Ramezani | en |
| Author | Azadeh Mohammadgholi | en |
| Orcid | Ardalan Montazeri [0000-0003-0011-4852] | en |
| Orcid | Mina Ramezani [0000-0002-9982-1276] | en |
| Orcid | Azadeh Mohammadgholi [0000-0002-5012-3768] | en |
| Issued Date | 2021-08-31 | en |
| Abstract | Background: Bromelain (BL) is an enzyme extracted from Ananas comosus, which has been known for its therapeutic properties. Objectives: The anticarcinogenic activity of BL was examined with and without the presence of magnetic carbon nanotubes (MCNTs) against HT-29 colorectal cancer cells. Methods: The operational factors affecting BL adsorption, such as contact time (30, 60, 90, 120, and 180 min), adsorbent dosage (1 g/L and 5 g/L), initial bromelain concentration (50, 150, and 300 mg/L), and temperature (35 and 50°C) were studied in details. Then, cancer cells were exposed to various BL concentrations (0.1, 1, 10, and 100 μg/mL), and the cell viability was determined by methylthiazol tetrazolium (MTT) assay after 24, 48, and 72 h. Results: The highest adsorption of BL on nanotubes was at 41.62 mg/L and achieved at 35°C and 90 min at the initial concentration of 50 mg/L and 1 g/L of MCNTs. The adsorption followed the Freundlich model and second-order kinetics. The results indicated that MCNTs could be a potential effective adsorbent for the removal of BL. Conclusions: MTT assay indicated that BL at a concentration of 100 μg/mL alone and in combination with MCNTs efficiently inhibited the HT-29 cancerous cells. However, encapsulated BL had a considerable advantage of slow delivery, which is favorable for cancer treatment. | en |
| DOI | https://doi.org/10.5812/jjnpp.108796 | en |
| URI | https://brieflands.com/journals/jjnpp/articles/108796 | en |
| Keyword | Bromelain Enzyme | en |
| Keyword | Anticarcinogenic | en |
| Keyword | Cytotoxicity | en |
| Keyword | Magnetic Carbon Nano-tubes | en |
| Keyword | Colorectal Cancer | en |
| Publisher | Brieflands | en |
| Title | Investigation the Effect of Encapsulated Bromelain Enzyme in Magnetic Carbon Nanotubes on Colorectal Cancer Cells | en |
| Type | Research Article | en |