Inhibitory effect of BMAP-28 on Leptospiral Lipopolysaccharide-Induced TLR2-Dependent Immune Response in Bovine Cells
| Author | Yijie GUO | en |
| Author | Cuiping Ding | en |
| Author | Bo Zhang | en |
| Author | Jun XU | en |
| Author | Meng XUN | en |
| Author | Jiru XU | en |
| Issued Date | 2016-06-30 | en |
| Abstract | Background: Bovine leptospirosis is a widespread zoonotic disease, leading to serious economic losses in animal production and causing potential hazards to human health. Leptospiral lipopolysaccharide (L-LPS) plays an important role in leptospirosis pathogenicity. Objectives: With respect to L-LPS endotoxin-like activity, we examined bovine immune response to L-LPS and the inhibitory ability of bovine myeloid antimicrobial peptide-28 (BMAP-28) against L-LPS-induced immune activation in bovine cells. Materials and Methods: In this study, L-LPS-induced proinflammatory cytokine production in bovine cells was quantitatively measured with real-time PCR and ELISA, and we determined which cell membrane receptors (toll-like receptor [TLR]2 and TLR4) played a major role. In addition, the ability of BMAP-28 to inhibit L-LPS-induced endotoxin-like immune activation in bovine cells was determined by the decrease in cytokine secretion. Results: L-LPS showed the ability to induce cytokine production in bovine cells, and its induction was TLR2-dependent. BMAP-28 was used to inhibit L-LPS-induced endotoxin-like activity. The function of BMAP-28 was to inhibit LPS-induced TLR2 expression and cytokine production. Conclusions: In this study, the L-LPS immune response of bovine cells was significant, indicating that TLR2 is the predominant receptor for L-LPS. Due to L-LPS endotoxin-like activity, we found a strategy through using BMAP-28 to prevent L-LPS-induced TLR2-dependent immune activation in bovine cells. | en |
| DOI | https://doi.org/10.5812/jjm.33926 | en |
| Keyword | LPS | en |
| Keyword | Toll-Like Receptor 2 | en |
| Keyword | Cytokines | en |
| Keyword | BMAP-28 | en |
| Keyword | Cattle | en |
| Keyword | <i>Leptospira</i> | en |
| Publisher | Brieflands | en |
| Title | Inhibitory effect of BMAP-28 on Leptospiral Lipopolysaccharide-Induced TLR2-Dependent Immune Response in Bovine Cells | en |
| Type | Research Article | en |
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