Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule
| Author | Xian Yang | en |
| Author | Shui-Ping Yang | en |
| Author | Xue Zhang | en |
| Author | Xiao-Dong Yu | en |
| Author | Qi-Yi He | en |
| Author | Bo-Chu Wang | en |
| Issued Date | 2014-10-31 | en |
| Abstract | The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable. | en |
| DOI | https://doi.org/10.22037/ijpr.2014.1595 | en |
| Keyword | The multiple indicator | en |
| Keyword | HPLC fingerprint | en |
| Keyword | Wuwei Changyanning granule | en |
| Keyword | Common peaks number | en |
| Keyword | The percentage of non-common peaks and similarity. | en |
| Publisher | Brieflands | en |
| Title | Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule | en |
| Type | Original Article | en |