Effects of Methanol Extract of Green Tea on Biofilm Formation of <i>Staphylococcus aureus</i>

Abstract

Background and Objectives: The aim of this study was to investigate the effect of ethanol extract of green tea on biofilm formation of Staphylococcus aureus. Methods: Staphylococcus aureus samples were obtained from hospital sources and plant extracts; green tea was made using the rotary. The minimum inhibitory concentration was determined using the microdilution method. Inhibition of biofilm formation was determined by using a microplate. Results: The results of this study showed that the greatest resistance was to the antibiotics penicillin (88.2%) and erythromycin (82.4%) and the least resistance to the antibiotic Amikacin (76/11%), respectively. The results of the effect of green tea extract showed the lowest inhibitory concentration (MIC) is equal to 0.3 milligrams per milliliter, which is a strain on the inhibitory concentration, and the highest concentration (MBC) MBC resistant Staphylococcus aureus equal to 20 milligrams per milliliter, which is controlled unidirectional. The results of green tea extract effects on biofilm showed that with increasing concentrations of less biofilm formation the biofilm formation has been much lower concentration of the extract. Conclusions: The results of this study indicate good effects of green tea extract to inhibit biofilm formation of Staphylococcus aureus, which can be used to treat infections caused by these bacteria’s.