Inhibitory Effects of Cichorium Intybus Root Extract on the Proliferation and Lipogenesis of the SKBR3 Cells
| Author | Reza Mehrandish | en |
| Author | Nasrin Dehghan-Nayeri | en |
| Author | Aliasghar Rahimian | en |
| Author | Ali Toutounchi | en |
| Author | Ali Mellati | en |
| Issued Date | 2018-04-30 | en |
| Abstract | Background: Fatty acid synthase (FASN) is a key enzyme in de novo lipogenesis pathway. FASN overexpression is a common feature of many human cancers like breast cancer. Furthermore, FASN expression in HER2 - positive cell lines like SKBR3 is more than other cell lines, such as MCF - 7, which are not HER2 - positive. Cichorium intybus is a medicinal herb and methanolic extract of this plant significantly suppressed cell viability and growth in some cancer cells. Objectives: In this study, we aimed at investigating the effect of methanolic extract of Cichorium intybus on the FASN expression and, therefore, lipogenesis pathway in human breast cancer SKBR3 cell line. Methods: We assessed the cytotoxicity effect of Chicorium intybus on the cell viability of SKBR3 cells, using MTT assay. In addition, apoptosis rate was assessed by annexinV/PI flow cytometry. Finally, Real time q - PCR was used for the analysis of FASN gene expression. Results: The results showed that the methanolic extract of Cichorium intybus caused a dose - dependent decrease in the cell viability of SKBR3 cells. Additionally, the treatment of confluent SKBR3 cells with extract led to reduced FASN expression at mRNA level. Conclusions: These results suggest that Chicorium intybus not only inhibits cell viability in a dose - dependent manner, but also presumably inhibits lipogenesis by markedly decreased FASN expression as a key lipogenic enzyme. | en |
| DOI | https://doi.org/10.5812/ijcm.9436 | en |
| URI | https://brieflands.com/journals/ijcm/articles/9436 | en |
| Keyword | Fatty Acid Synthase | en |
| Keyword | Cichorium Intybus | en |
| Keyword | SKBR3 Cells | en |
| Keyword | Anti - Proliferation | en |
| Keyword | Lipogenesis Pathway | en |
| Publisher | Brieflands | en |
| Title | Inhibitory Effects of Cichorium Intybus Root Extract on the Proliferation and Lipogenesis of the SKBR3 Cells | en |
| Type | Research Article | en |
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