Total Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of<i> Artemisia biennis </i>Willd

AuthorTayyebe Hatamien
AuthorSayyed Ahmad Emamien
AuthorSayyed Shahram Miraghaeeen
AuthorMahdi Mojarraben
Issued Date2014-04-30en
AbstractTotal phenolic contents (TPC) of five different extracts (petroleum ether, dichloromethane, ethyl acetate, ethanol and ethanol-water) of Artemisia biennis Willd were measured in this work. The antioxidant activity was investigated by three different methods: β-carotene bleaching (BCB) test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and ferrous ion chelating (FIC) assay. Among all the extracts analyzed, the hydroethanolic extract exhibited a significantly higher phenolic content and antioxidant activity than other samples. Vacuum liquid chromatography of this extract yielded seven fractions (A to G) which were subjected to all aforementioned experiments. The highest total phenolic content and free radical scavenging activites were present in the same sample (Fraction D) but the only statistically significant correlation between TPC and EC50 values was observed for BCB.en
DOIhttps://doi.org/10.22037/ijpr.2014.1518en
Keyword<i>Artemisia biennis</i>en
KeywordTotal phenolic contenten
KeywordAntioxidant activityen
PublisherBrieflandsen
TitleTotal Phenolic Contents and Antioxidant Activities of Different Extracts and Fractions from the Aerial Parts of<i> Artemisia biennis </i>Willden
TypeOriginal Articleen

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