Potential Anti-Tumor Activity of Kefir-Induced Juglone and Resveratrol Fractions Against Ehrlich Ascites Carcinoma-Bearing BALB/C Mice
| Author | Erhan Bozkurt | en |
| Author | Emre Atay | en |
| Author | Gökhan Pektaş | en |
| Author | Ayşe Ertekin | en |
| Author | Ayhan Vurmaz | en |
| Author | Ömer Adil Korkmaz | en |
| Author | Gökhan Sadi | en |
| Author | Esra Aslan | en |
| Author | Oğuz Han Koca | en |
| Author | Mehmet Bilgehan Pektaş | en |
| Issued Date | 2020-07-31 | en |
| Abstract | We investigated the potential influence of kefir-induced juglone and resveratrol fractions (JRK) against Ehrlich Ascites Carcinoma (EAC) bearing BALB/c male mice. Kefir yeast was grown in the cell culture supplemented with juglone and resveratrol (1:2). After 48 h incubation, JRK solution was applied (0.1 mL/day i.p.) to the EAC-bearing mice throughout five days. Molecular regulatory mechanisms of apoptotic and anti-apoptotic pathway components were evaluated in the plasma of mice and isolated EAC cells with ELISA, qRT-PCR, and immunocytchemical experiments. EAC-induced upregulation in Bcl-2 and downregulation in Caspase-3 were normalized with JRK in the plasma of mice. Additionally, JRK upregulated the expression levels of apoptotic Bax, p53, Caspase-3,8,9, and APAF-1 proteins together with BAX, CASPASE-8, and CASPASE-9 genes in isolated EAC cells. These changes were also associated with decreased expression levels of anti-apoptotic Bcl-2 and Bcl-xl proteins. Immunocytochemical studies also confirmed the activation of apoptotic pathways and repression of anti-apoptotic proteins in EAC cells with JRK treatment. JRK activates apoptotic pathway and inhibits anti-apoptotic genes and proteins in Ehrlich ascites carcinoma- bearing BALB/c mice that could be beneficial in cancer treatment. | en |
| DOI | https://doi.org/10.22037/ijpr.2020.112993.14060 | en |
| Keyword | Juglone | en |
| Keyword | Resveratrol | en |
| Keyword | Kefir | en |
| Keyword | Ehrlich ascites | en |
| Keyword | Bax | en |
| Keyword | Bcl-2 | en |
| Keyword | p53 | en |
| Keyword | Caspase | en |
| Publisher | Brieflands | en |
| Title | Potential Anti-Tumor Activity of Kefir-Induced Juglone and Resveratrol Fractions Against Ehrlich Ascites Carcinoma-Bearing BALB/C Mice | en |
| Type | Original Article | en |
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