Anti-Mutagenicity and Apoptotic Effects of <i>Teucrium polium</i> L. Essential Oil in HT29 Cell Line

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AuthorFataneh Hashem-Dabaghianen
AuthorAsie Shojaiien
AuthorJinous Asgarpanahen
AuthorMaliheh Entezarien
OrcidAsie Shojaii [0000-0002-9093-5525]en
OrcidMaliheh Entezari [0000-0003-4191-3351]en
Issued Date2020-08-31en
AbstractObjectives: Regarding the high prevalence of cancer in Iran and the cytotoxic properties of T. polium, the current study aimed to investigate the cytotoxic and anti-mutagenicity effect of T. polium essential oil (TpEO) on human colon adenocarcinoma cell line (HT29). Methods: HT29 cells were cultured in L-glutamine, RPMI Sigma (1640), with 10% of FBS (fetal bovine serum). Then, the cultures were incubated with 5% CO2 at 37°C, and MTT assay was used to recognize cell proliferation under the inhibitory influence of TpEO. The cell cycle progression was monitored by Sub-G1 apoptosis assay using flow cytometry. Eventually, the anti-mutagenicity property was evaluated using the Ames test employing TA100 and exposure to sodium azide as the carcinogenic substance. Results: The cytotoxic effect of TpEO on HT29 cells was 66.867 ± 1.37 µg/mL. Cultured HT29 cells treated with TpEO exhibited morphological features of apoptosis. TpEO preventive effect was about 78.18%. Conclusions: This study showed that TpEO may be useful for treating colon cancer.en
DOIhttps://doi.org/10.5812/jjnpp.79559en
Keyword<i>Teucrium polium</i>en
KeywordPersian Medicineen
KeywordHerbal Medicineen
KeywordHT29 Cell Lineen
KeywordMutagenicityen
KeywordAnti-Neoplastic Agentsen
PublisherBrieflandsen
TitleAnti-Mutagenicity and Apoptotic Effects of <i>Teucrium polium</i> L. Essential Oil in HT29 Cell Lineen
TypeResearch Articleen

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