Investigating the Expression Level of circPVT1 and miR-203a-3p in Tissue Samples of Patients with Gastric Cancer
| Author | Saba Asadi | en |
| Author | Neda Mousavi-Niri | en |
| Author | Leili Rejali | en |
| Author | Mehrdad Hashemi | en |
| Orcid | Saba Asadi [0009-0004-1232-3517] | en |
| Orcid | Neda Mousavi-Niri [0000-0001-5136-2122] | en |
| Orcid | Leili Rejali [0000-0002-4194-373X] | en |
| Orcid | Mehrdad Hashemi [0000-0003-0627-6991] | en |
| Issued Date | 2025-12-31 | en |
| Abstract | Background: Gastric carcinoma is among the most prevalent and aggressive cancers of the digestive tract, with early detection being essential for improving patient outcomes and survival rates. Objectives: This research aimed to assess the potential of hsa_circPVT1 and miR-203a-3p as molecular biomarkers for gastric cancer (GC). Methods: Twenty-five gastric tumor samples and paired adjacent normal tissues were retrospectively collected from patients with gastric adenocarcinoma, with diagnoses confirmed by specialists. The cohort included 18 males and 7 females, mostly aged 65 or older, with tumor stages ranging from IA to IV. RNA extraction and complementary DNA (cDNA) synthesis were performed, followed by quantification of hsa_circPVT1 and miR-203a-3p expression using real-time PCR; indeterminate results were retested. Analyses were conducted on stored tissues without clinical intervention, with minimal time between tests, and no adverse events were reported. Diagnostic accuracy was assessed by ROC curve analysis using optimized cut-offs. All analyses were blinded to clinical and pathological data to minimize bias. Results: The hsa_circPVT1 expression was significantly higher in tumors compared to adjacent normal tissues, with cross-tabulation confirming this difference. The miR-203a-3p showed no clear distinction. ROC analysis demonstrated strong diagnostic accuracy for hsa_circPVT1 [area under the curve (AUC) 0.84, 95% CI 0.65 - 1.00], but limited performance for miR-203a-3p (AUC 0.69, 95% CI 0.45 - 0.93). Conclusions: These findings indicate that hsa_circPVT1 could be a valuable diagnostic biomarker and a potential therapeutic target for gastric carcinoma, whereas miR-203a-3p may have limited standalone diagnostic relevance. | en |
| DOI | https://doi.org/10.5812/ijcm-160277 | en |
| Keyword | circPVT1 | en |
| Keyword | miR-203a-3p | en |
| Keyword | Gastric Cancer | en |
| Keyword | Biomarker | en |
| Keyword | Molecular Diagnostics | en |
| Keyword | ROC Curve | en |
| Keyword | AUC | en |
| Keyword | Diagnostic Accuracy | en |
| Keyword | Retrospective Study | en |
| Publisher | Brieflands | en |
| Title | Investigating the Expression Level of circPVT1 and miR-203a-3p in Tissue Samples of Patients with Gastric Cancer | en |
| Type | Research Article | en |
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