Structural Characterisation of a Polysaccharide from Radix Ranunculus Ternati
| Author | Xuefeng Huang | en |
| Author | Yun Zhao | en |
| Author | Xin Jin | en |
| Issued Date | 2014-10-31 | en |
| Abstract | A water soluble polysaccharide, HB-1, with a molecular weight of 23,930, was isolated from radix Ranunculi ternati. by hot water extraction, ethanol precipitation, deproteination，ultrafiltration and gel-filtration column chromatography. Its sugar composition was determined by GLC as Glc, Ara, and Gal in a molar ration of 16.071: 2.722: 1. And the absolute configuration of Glc was identified as D. Smith degradation and methylation reaction showed the proportion of —1Glc (A) was about 16%, —1Glc4— (B) about 62%, (C) about 14%, and —1Gal6— (D) about 8%. The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D. Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit. The inhibition activity of HB-1 on human glioma cell line SF188 was also measured, only to find it inactive. | en |
| DOI | https://doi.org/10.22037/ijpr.2014.1564 | en |
| Keyword | Ranunculus ternatus Thunb | en |
| Keyword | Polysaccharide | en |
| Keyword | Ultrafiltration | en |
| Keyword | Absolute configuration | en |
| Keyword | Smith degradation | en |
| Keyword | Methylation reaction | en |
| Keyword | Anti-tumor activity | en |
| Publisher | Brieflands | en |
| Title | Structural Characterisation of a Polysaccharide from Radix Ranunculus Ternati | en |
| Type | Original Article | en |
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