17β-Estradiol Stimulates Generation of Reactive Species Oxygen and Nitric Oxide in Ovarian Adenocarcinoma Cells (OVCAR 3)
| Author | Jafar Maleki | en |
| Author | Mitra Nourbakhsh | en |
| Author | Mohammad Shabani | en |
| Author | Mohsen Korani | en |
| Author | Seyed Manuchehr Nourazarian | en |
| Author | Mohammad Reza Ostadali Dahaghi | en |
| Author | Mohamad Hossein Moghadasi | en |
| Issued Date | 2015-05-28 | en |
| Abstract | Background:: Experimental and epidemiological evidence supports a role for steroid hormones in the pathogenesis of ovarian cancer. Among steroid hormones, 17β-estradiol (E2) has the most potent effect on proliferation, apoptosis and metastasis. Objectives:: In the present study, we investigated the effect of E2 on production of ROS and NO in ovarian cancer cells. Materials and Methods:: Ovarian adenocarcinoma cell line (OVCAR-3) was cultured and treated with various concentrations of E2, antioxidants (N-acetyle cysteine and Ebselen) and ICI182780 as an estrogen receptor antagonist. MTT test was performed to evaluate cell viability. NO and ROS levels were measured by Griess and DCFH-DA methods, respectively. Results:: ROS levels as well as NO levels were increased in OVCAR-3 cells treated with E2. The increase in ROS production was in parallel with increased cell viability which indicates that estrogen-induced ROS can participate in cancer progression. ICI182780 abolished E2-induced ROS production. Progesterone was also effective in reducing ROS and NO generation. Conclusions:: NO and ROS are important molecules in signaling networks in cell. These molecules can be used as therapeutic targets for prevention and treatment of ovary cancer and other estrogen-induced malignancies. | en |
| DOI | https://doi.org/10.17795/ijcp2332 | en |
| Keyword | Reactive Oxygen Species | en |
| Keyword | 17β-estradiol | en |
| Keyword | Ovarian Adenocarcinoma Cells | en |
| Keyword | DCFH-DA | en |
| Publisher | Brieflands | en |
| Title | 17β-Estradiol Stimulates Generation of Reactive Species Oxygen and Nitric Oxide in Ovarian Adenocarcinoma Cells (OVCAR 3) | en |
| Type | Research Article | en |
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