Evaluation of Sensitivity of Molecular Methods for Detection of Rifampin-Resistant Strains Amongst Drug-resistant <i>Mycobacterium tuberculosis</i> Isolates
| Author | Mahdi Behdani | en |
| Author | Hoshang Rafatpanah | en |
| Author | Alireza Mohammadzadeh | en |
| Author | Kiarash Ghazvini | en |
| Author | Mostafa Amiri | en |
| Author | Jalal Mardaneh | en |
| Issued Date | 2017-07-31 | en |
| Abstract | Background: Resistance to rifampin in multidrug-resistant M. tuberculosis (MDR-TB) is one of the major threats to the global public health system. Objectives: The aim of the present study was to explore the molecular characterization of resistance against rifampin amongst multidrug-resistant (MDR) and extensively drug-resistant (XDR) isolates obtained from patients. Methods: In this study, we used XDR (n = 6), MDR (n = 9) and rifampin sensitive (n = 39) isolates whose drug susceptibility patterns were identified by proportion method. A simple single-step multiplex-allele specific-polymerase chain reaction (MAS-PCR) assay was optimized to detect the three most frequent mutations in rifampin resistance-determining region (RRDR) fragment of rpoB gene. Additionally, single-strand conformational polymorphism (SSCP)-PCR and Sequencing were utilized. Results: Out of nine MDR isolates, five were detected by MAS-PCR as rifampin resistant (sensitivity; 55.5%). In comparison with the proportion method, the sensitivity of SSCP for XDR, MDR and rifampin sensitive isolates were 83.3%, 77.7% and 97.4%, respectively. The DNA sequencing revealed that three of the 6 XDR isolates had several silent mutations, one isolate had one silent mutation, one strain had no mutations and only one isolate had mutations at codon 531. Conclusions: In sum, although the molecular methods are rapid, they are not able to identify resistance against rifampin efficiently. | en |
| DOI | https://doi.org/10.5812/pedinfect.40580 | en |
| Keyword | <i>Mycobacterium tuberculosis</i> | en |
| Keyword | Multidrug-Resistant | en |
| Keyword | Extensively Drug-Resistant | en |
| Keyword | Rifampin | en |
| Publisher | Brieflands | en |
| Title | Evaluation of Sensitivity of Molecular Methods for Detection of Rifampin-Resistant Strains Amongst Drug-resistant <i>Mycobacterium tuberculosis</i> Isolates | en |
| Type | Research Article | en |