Acrylamide Induced Oxidative Cellular Senescence in Embryonic Fibroblast Cell Line (NIH 3T3): A Protection by Carvacrol
| Author | Mehdi Evazalipour | en |
| Author | Pouya Safarzadeh Kozani | en |
| Author | Pooria Safarzadeh Kozani | en |
| Author | Sahar Shabani | en |
| Author | Bahar Rezaei Soufi | en |
| Author | Ehsan Zamani | en |
| Orcid | Ehsan Zamani [0000-0003-2033-0683] | en |
| Issued Date | 2021-11-30 | en |
| Abstract | Background: Stress-induced cellular senescence is a perpetual state of cell cycle arrest occurring in proliferating cells in response to stressful conditions. It is believed that oxidative stress plays a unique role in this process. As a reactive chemical compound that can induce oxidative stress, acrylamide is widely applied in several fields. Carvacrol is a liquid phenolic monoterpenoid found in essential oils of some plants and is known for its antioxidant and anticarcinogenic properties. Objectives: The current study aimed to evaluate the effects of carvacrol on oxidative stress and cellular senescence induced by acrylamide in the NIH 3T3 cell line. Methods: NIH 3T3 embryonic fibroblast cells were exposed to different concentrations of acrylamide, carvacrol, and H2O2 in a cell culture medium. The level of β-galactosidase (SA-β-gal) activity, as a marker of cellular senescence, was measured using staining and quantitative assays. Furthermore, to measure oxidative stress parameters, the content of glutathione and lipid peroxidation were determined. Results: Acrylamide could induce premature senescence evident by the elevated lipid peroxidation and SA-β-gal activity and declined cell viability and glutathione. Moreover, carvacrol showed beneficial effects on both acrylamide- and H2O2-induced cellular senescence by significantly reversing or subsiding the effect of oxidative stress and mediating its consequences. Conclusions: It can be concluded that carvacrol has protective effects against oxidative cellular senescence induced by acrylamide in the NIH 3T3 cell line. | en |
| DOI | https://doi.org/10.5812/jjnpp.109399 | en |
| Keyword | Acrylamide | en |
| Keyword | Carvacrol | en |
| Keyword | Oxidative Stress | en |
| Keyword | Senescence | en |
| Keyword | Fibroblasts | en |
| Publisher | Brieflands | en |
| Title | Acrylamide Induced Oxidative Cellular Senescence in Embryonic Fibroblast Cell Line (NIH 3T3): A Protection by Carvacrol | en |
| Type | Research Article | en |