Study of CCR5-59353C/T Polymorphism in the Iranian Patients With Chronic Hepatitis B Virus Infection
| Author | Farahnaz Bineshian | en |
| Author | Sajjad Jalali | en |
| Author | Zohreh Sharifi | en |
| Orcid | Zohreh Sharifi [0000-0001-6297-7659] | en |
| Issued Date | 2017-10-31 | en |
| Abstract | Background: CCR5 is identified as one of the most important chemokine receptors with a major role in the creation of chemotaxis and mobilization of immunocompetent cells and moving them toward the liver for thorough cleaning of the virus. CCR5-59353 (C/T) is an important promoter polymorphism of chemokine receptor 5. Some studies showed a relationship between CCR5-59353 (C/T) polymorphism and clearance or persistence of hepatitis B virus (HBV) infection. Objectives: The current study aimed at developing polymorphism CCR5-59353 (C/T) in Iranian patients with chronic HBV infection. Methods: A total of 200 blood samples including 100 healthy controls and 100 HBsAg-positive patients were randomly selected. Samples were tested for HBsAg by the enzyme-linked immunosorbent assay (ELISA) and HBV-DNA by the polymerase chain reaction (PCR) method. Genomic DNA was extracted from blood buffy coat using the salting out method. CCR5-59353 (C/T) polymorphism was genotyped by the allele specific amplification (ASA) PCR. Chi-square test was used for statistical analysis. Results: Five percent of control samples and 12% of patient samples had CC mutant genotype. Nevertheless, there was no significant difference in genotypes frequency of CCR5-59353 between the groups (P = 0.1). Conclusions: It seems that CCR5-59353 polymorphism was not associated with chronic HBV infection outcome in the Iranian population. However, the frequency of CC genotype was higher in the patient group (12%) than the control group (5%). | en |
| DOI | https://doi.org/10.5812/mejrh.12658 | en |
| URI | https://brieflands.com/journals/mejrh/articles/12658 | en |
| Keyword | Polymorphism | en |
| Keyword | CCR5-59353C/T | en |
| Keyword | Chronic HBV | en |
| Keyword | Infection | en |
| Publisher | Brieflands | en |
| Title | Study of CCR5-59353C/T Polymorphism in the Iranian Patients With Chronic Hepatitis B Virus Infection | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- mejrh-04-04-12658.pdf
- Size:
- 154.64 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF