Cytotoxic Activity and Phytochemical Analysis of <i>Artemisia biennis </i>Essential Oils at Different Growth Stages
| Author | Nasim Jamshidi | en |
| Author | Mahdi Mojarrab | en |
| Author | Mohammad-Reza Delnavazi | en |
| Author | Ahmad Emami | en |
| Author | Gholamreza Bahrami | en |
| Author | Yalda Shokoohinia | en |
| Author | Leila Hosseinzadeh | en |
| Orcid | Mahdi Mojarrab [0000-0002-9771-5373] | en |
| Orcid | Mohammad-Reza Delnavazi [0000-0002-8127-9714] | en |
| Orcid | Ahmad Emami [0000-0003-4298-3132] | en |
| Orcid | Gholamreza Bahrami [0000-0002-5917-3040] | en |
| Orcid | Leila Hosseinzadeh [0000-0003-0536-3209] | en |
| Issued Date | 2024-11-30 | en |
| Abstract | Background: The Artemisia genus is well-known for its medicinal properties, particularly in Iranian traditional medicine. Artemisia biennis, a species within this genus, is widely distributed across Iranian rangelands. Objectives: This study aimed to evaluate the cytotoxic activity and chemical composition of essential oils (EO) isolated from the aerial parts of A. biennis in Iran at different growth stages. Methods: The aerial parts of A. biennis from northeast Iran were collected during June (early vegetative stage), July (pre-flowering stage), August (full-flowering stage), and October (late vegetative stage). The essential oils (EOs) of the A. biennis species were extracted by hydro-distillation using a Clevenger-type apparatus and analyzed using gas chromatography–mass spectrometry (GC/MS). The cytotoxic activity of the EOs against normal fibroblasts, MCF-7, and HT-29 cell lines was evaluated using the MTT assay. Results: Data from GC/MS analysis revealed that (Z)-nerolidol (22.62 - 54.4%), (E)-β-farnesene (6.89-16.38%), and (Z)-tonghaosu (12.33 - 18.61%) were the most abundant chemical constituents. The essential oil (EO) of A. biennis was characterized by a high content of oxygenated sesquiterpenes, with (Z)-nerolidol being the main constituent identified. Based on the MTT assay, the EO of the plant species collected in June exhibited the most potent cytotoxicity against MCF-7 (IC50 = 2.84 ± 0.15 µg/mL) and HT-29 cell lines (IC50 = 2.41 ± 0.2 µg/mL). Conclusions: The growth stage of A. biennis affects EO yields, the composition of extracted secondary metabolites, and cytotoxic activity. A. biennis EOs can be considered potential sources of cytotoxic phytochemicals. | en |
| DOI | https://doi.org/10.5812/jjnpp-147988 | en |
| URI | https://brieflands.com/journals/jjnpp/articles/147988 | en |
| Keyword | <i>Artemisia biennis</i> | en |
| Keyword | Growth Stages | en |
| Keyword | Nerolidol | en |
| Keyword | Tonghaosu | en |
| Keyword | Farnesene | en |
| Keyword | Cytotoxic Activity | en |
| Publisher | Brieflands | en |
| Title | Cytotoxic Activity and Phytochemical Analysis of <i>Artemisia biennis </i>Essential Oils at Different Growth Stages | en |
| Type | Research Article | en |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- jjnpp-19-4-147988-publish-pdf.pdf
- Size:
- 750.56 KB
- Format:
- Adobe Portable Document Format
- Description:
- Article/s PDF