Molecular Mechanisms of Luteolin Induced Growth Inhibition and Apoptosis of Human Osteosarcoma Cells
| Author | Yonghong Wang | en |
| Author | Daliang Kong | en |
| Author | Xinwei Wang | en |
| Author | Xiaoxiong Dong | en |
| Author | Yingying Tao | en |
| Author | Haiyang Gong | en |
| Issued Date | 2015-04-30 | en |
| Abstract | Luteolin is a flavone in medicinal plants as well as some vegetables and spices. It is a natural anti-oxidant with less pro-oxidant potential but apparently with a better safety profile. The purpose of this study was to investigate the molecular mechanism of luteolin-mediated apoptosis of MG-63 human osteosarcoma cells. MTT assay kit was employed to evaluate the effects of luteolin on MG-63 cells proliferation. Then, we performed Annexin V-FITC/PI to analyze the apoptotic rate of the cells. Furthermore, the inhibitory effects of luteolin on the expressions of BCL-2, BAX, Caspase-3 and Survivin were detected by Western blotting. As expected, luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the growth of MG-63 cells by inhibiting cell proliferation and inducing cell apoptosis. Western blotting demonstrated that luteolin (0.5, 2.5, 12.5 µg/mL) inhibited the expressions of BCL-2, Caspase-3 and Survivin, and promoted the expression of BAX in MG-63 cells with a concentration dependent way. Luteolin can inhibit osteosarcoma cell proliferation and induce apoptosis effectively in a dose dependent manner through down-regulating the expression of BCL-2, Caspase-3 and Survivin proteins levels and up-regulating the expression of BAX protein level. These findings indicated that luteolin may be used as a novel herbal medicine for the treatment of osteosarcoma. | en |
| DOI | https://doi.org/10.22037/ijpr.2015.1670 | en |
| URI | https://brieflands.com/journals/ijpr/articles/125338 | en |
| Keyword | Luteolin | en |
| Keyword | Osteosarcoma cells | en |
| Keyword | Proliferation | en |
| Keyword | Apoptosis | en |
| Keyword | Anti-oxidant | en |
| Publisher | Brieflands | en |
| Title | Molecular Mechanisms of Luteolin Induced Growth Inhibition and Apoptosis of Human Osteosarcoma Cells | en |
| Type | Original Article | en |
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